In the last decade, the benthic dinoflagellate Ostreopsis ovata has caused severe sanitary emergencies along the Italian coasts. Liquid chromatography-mass spectrometry (LC-MS) played a key role in the identification of putative palytoxin as the parent toxin produced by O. ovata. Following this finding, a number of palytoxin analogues, named ovatoxin-a, -b, -c, -d, and -e, have been found and identified basing on high resolution (HR) LC-MS evidence. Among them, ovatoxin-a is the major component of O. ovata toxin profile, representing up to 55% of the total toxin content, with the remaining 46% being constituted by the whole of the other ovatoxins and putative palytoxin. HR LC-MS studies on O. ovata are continuing. Toxin profiles of different O. ovata strains appear sometimes different both qualitatively and quantitatively and the presence of further new ovatoxins is emerging. In addition, preliminary information on the structure of ovatoxins has been gained by an in-depth investigation of their HR LC-MS/MS spectra in comparison to that of palytoxin. A comparison between HR LC-MS/MS spectra of palytoxin and ovatoxin-a allowed us to individuate the regions and, in some cases, the sites where structural differences between these molecules occur. In view of the structural complexity of ovatoxins, the fragmentation pattern of palytoxin and ovatoxin-a could serve as useful template to characterize minor congeners that could be available in too small amounts to be studied by NMR.
High Resolution LC-MS and MS/MS studies of Ostreopsis ovata toxin profile / Dell'Aversano, Carmela; Ciminiello, Patrizia; DELLO IACOVO, Emma; Fattorusso, Ernesto; Forino, Martino; Tartaglione, Luciana. - (2011). (Intervento presentato al convegno Mycotoxin and Phycotoxins, Gordon Research Seminar and Conference tenutosi a Colby College, Waterville, ME, USA nel 11-17/6/2011).
High Resolution LC-MS and MS/MS studies of Ostreopsis ovata toxin profile
DELL'AVERSANO, CARMELA;CIMINIELLO, PATRIZIA;DELLO IACOVO, EMMA;FATTORUSSO, ERNESTO;FORINO, MARTINO;TARTAGLIONE, LUCIANA
2011
Abstract
In the last decade, the benthic dinoflagellate Ostreopsis ovata has caused severe sanitary emergencies along the Italian coasts. Liquid chromatography-mass spectrometry (LC-MS) played a key role in the identification of putative palytoxin as the parent toxin produced by O. ovata. Following this finding, a number of palytoxin analogues, named ovatoxin-a, -b, -c, -d, and -e, have been found and identified basing on high resolution (HR) LC-MS evidence. Among them, ovatoxin-a is the major component of O. ovata toxin profile, representing up to 55% of the total toxin content, with the remaining 46% being constituted by the whole of the other ovatoxins and putative palytoxin. HR LC-MS studies on O. ovata are continuing. Toxin profiles of different O. ovata strains appear sometimes different both qualitatively and quantitatively and the presence of further new ovatoxins is emerging. In addition, preliminary information on the structure of ovatoxins has been gained by an in-depth investigation of their HR LC-MS/MS spectra in comparison to that of palytoxin. A comparison between HR LC-MS/MS spectra of palytoxin and ovatoxin-a allowed us to individuate the regions and, in some cases, the sites where structural differences between these molecules occur. In view of the structural complexity of ovatoxins, the fragmentation pattern of palytoxin and ovatoxin-a could serve as useful template to characterize minor congeners that could be available in too small amounts to be studied by NMR.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
