Scenedesmus strains have been frequently selected for mass culture and for their potential applications in biofuel production . However, a key aspect for the widespread of microalgae large-scale production is the development of microalgal-based biorefineries to produce a wider range of products that altogether may contribute to the economic potential of the enterprise. In order to reduce the costs of algal-based biofuel production we suggest to combine wastewater treatment and oil production. At the University of Napoli, 110 algal strains selected from the ACUF collection at the University of Napoli (ACUF, www.biologiavegetale.unina.it/acuf.html) have been previously characterised for their extracellular phenoloxidase enzymatic activity. Among them, Scenedesmus vacuolatus (ACUF-095) showed the best performance. Furthermore, the adoption of genetically modified S. vacuolatus strains in the phycoremediation of hazardous phenolic pollutants has been previously investigated. This contribution reports on optimal conditions of Scenedesmus growth with the aim to combine efficient nitrogen removal, high lipid production, and enhanced release of extracellular phenoloxidases in the culture medium. Seven S. vacuolatus strains were characterized for their ability to grow under different pH and salinity conditions. The most promising strains (ACUF-029 and ACUF-095) have been cultivated in 1 Liter lab-scale bubble column photobioreactors under continuous artificial light (I= 300 AE/(m2 s)). The strain 095 grown with air bubbling reached a biomass production of about 5 g/L, a phenoloxidase activity ranging from 2.85 to 3.93 U/ml, and lipid content was about 25% of dry weight. Under a 5% CO2 concentration in the gas phase, the strain 095 afforded higher biomass (10 g/L) and lipid productivity (about 34% under nitrogen low concentration), and maximum phenoloxidase activity. Lipid composition was also analysed after extraction and alkaline transesterification in terms of methyl-esters.
Scenedesmus vacuolatus as a cell factory for combined biodiesel production and extracellular phenoloxidase activity in lab-scale photobioreactors / Olivieri, Giuseppe; Andreozzi, Roberto; Marzocchella, Antonio; Pinto, Gabriele; Pollio, Antonino; Chiaiese, Pasquale; Filippone, Edgardo. - (2012). (Intervento presentato al convegno 2nd International Conference on Algal Biomass, Biofuels and Bioproducts tenutosi a San Diego (USA) nel 10-13 June 2012).
Scenedesmus vacuolatus as a cell factory for combined biodiesel production and extracellular phenoloxidase activity in lab-scale photobioreactors
OLIVIERI, GIUSEPPE;ANDREOZZI, ROBERTO;MARZOCCHELLA, ANTONIO;PINTO, GABRIELE;POLLIO, ANTONINO;CHIAIESE, Pasquale;FILIPPONE, EDGARDO
2012
Abstract
Scenedesmus strains have been frequently selected for mass culture and for their potential applications in biofuel production . However, a key aspect for the widespread of microalgae large-scale production is the development of microalgal-based biorefineries to produce a wider range of products that altogether may contribute to the economic potential of the enterprise. In order to reduce the costs of algal-based biofuel production we suggest to combine wastewater treatment and oil production. At the University of Napoli, 110 algal strains selected from the ACUF collection at the University of Napoli (ACUF, www.biologiavegetale.unina.it/acuf.html) have been previously characterised for their extracellular phenoloxidase enzymatic activity. Among them, Scenedesmus vacuolatus (ACUF-095) showed the best performance. Furthermore, the adoption of genetically modified S. vacuolatus strains in the phycoremediation of hazardous phenolic pollutants has been previously investigated. This contribution reports on optimal conditions of Scenedesmus growth with the aim to combine efficient nitrogen removal, high lipid production, and enhanced release of extracellular phenoloxidases in the culture medium. Seven S. vacuolatus strains were characterized for their ability to grow under different pH and salinity conditions. The most promising strains (ACUF-029 and ACUF-095) have been cultivated in 1 Liter lab-scale bubble column photobioreactors under continuous artificial light (I= 300 AE/(m2 s)). The strain 095 grown with air bubbling reached a biomass production of about 5 g/L, a phenoloxidase activity ranging from 2.85 to 3.93 U/ml, and lipid content was about 25% of dry weight. Under a 5% CO2 concentration in the gas phase, the strain 095 afforded higher biomass (10 g/L) and lipid productivity (about 34% under nitrogen low concentration), and maximum phenoloxidase activity. Lipid composition was also analysed after extraction and alkaline transesterification in terms of methyl-esters.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
