As partner of the ESA-funded program MELiSSA (Micro-Ecological Life Support System Alternative), the University of Naples conducted a series of experiments aimed to identify the best protocol for hydroponic cultivation of soybean [Glycine max (L.) Merr.], in the view of the use of higher plants for food production and resources regeneration in Bioregenerative Life Support Systems (BLSSs). When grown in open field, soybean plants normally establish specific root interactions with useful soil microorganisms. The aim of this study was to evaluate the effect of a microbial mix (Myco Madness, Humboldt nutrients), including 9 michorrhiza, 15 bacteria and 2 thrichoderma beneficial species, on plant performance of soybean cultivar ‘Pr91m10’ grown in Nutrient film technique (NFT) system. Inoculation was repeated at seed, seedlings and plant stages. Results reveal significantly higher values of plant height and leaf area in inoculated plants compared to control. Consistently, seed yield was significantly higher in inoculated plants. In terms of plant physiology, measurements of gas exchanges and chlorophyll fluorescence analyses showed higher net CO2 assimilation and transpiration rates in inoculated plants as well as higher values of photochemical efficiency, indicating a better photosynthetic performance in treated plants. Light and epi-fluorescence microscopy suggested that the modifications in eco-physiological behaviour are also due to alterations in anatomical traits, such as stomata size and frequency. The mechanisms underlying the positive influence of plant-microbe/s association was investigated through profiling of the microbial taxa of the rhizosphere microbiome. Finally the effect of the interaction between microbes and roots/plants on the nutritional value of seeds was evaluated through the analysis of proximate composition. In conclusion, inoculation with Myco Madness mix can be considered as a tool to improve the performance of soybean plants ‘Pr91m10’ in NFT, under controlled environment.
Ground-based research for soybean cultivation in BLSS: harmonization of plant structure and physiology in hydroponics / Paradiso, Roberta; DE MICCO, Veronica; Arena, Carmen; Aronne, Giovanna; DE PASCALE, Stefania. - (2015). (Intervento presentato al convegno ISLSWG Workshop On Bioregenerative Life Support tenutosi a Turin (Italy) nel 28-29 May).
Ground-based research for soybean cultivation in BLSS: harmonization of plant structure and physiology in hydroponics
PARADISO, ROBERTA;DE MICCO, VERONICA;ARENA, CARMEN;ARONNE, GIOVANNA;DE PASCALE, STEFANIA
2015
Abstract
As partner of the ESA-funded program MELiSSA (Micro-Ecological Life Support System Alternative), the University of Naples conducted a series of experiments aimed to identify the best protocol for hydroponic cultivation of soybean [Glycine max (L.) Merr.], in the view of the use of higher plants for food production and resources regeneration in Bioregenerative Life Support Systems (BLSSs). When grown in open field, soybean plants normally establish specific root interactions with useful soil microorganisms. The aim of this study was to evaluate the effect of a microbial mix (Myco Madness, Humboldt nutrients), including 9 michorrhiza, 15 bacteria and 2 thrichoderma beneficial species, on plant performance of soybean cultivar ‘Pr91m10’ grown in Nutrient film technique (NFT) system. Inoculation was repeated at seed, seedlings and plant stages. Results reveal significantly higher values of plant height and leaf area in inoculated plants compared to control. Consistently, seed yield was significantly higher in inoculated plants. In terms of plant physiology, measurements of gas exchanges and chlorophyll fluorescence analyses showed higher net CO2 assimilation and transpiration rates in inoculated plants as well as higher values of photochemical efficiency, indicating a better photosynthetic performance in treated plants. Light and epi-fluorescence microscopy suggested that the modifications in eco-physiological behaviour are also due to alterations in anatomical traits, such as stomata size and frequency. The mechanisms underlying the positive influence of plant-microbe/s association was investigated through profiling of the microbial taxa of the rhizosphere microbiome. Finally the effect of the interaction between microbes and roots/plants on the nutritional value of seeds was evaluated through the analysis of proximate composition. In conclusion, inoculation with Myco Madness mix can be considered as a tool to improve the performance of soybean plants ‘Pr91m10’ in NFT, under controlled environment.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
