Exploring the neuroprotective effects of synthetic compounds for new neurodrug development

Introduction: Alzheimer’s disease (AD) presents signifi cant therapeutic challenges, with amyloid β 1-42 (Aβ1-42) protein aggregation playing a central role in its pathogenesis. Aim: Our study investigates the potential therapeutic effi cacy of the isoindolinone derivative 3-(3-oxoisoindolin-1-yl)pentane-2,4-dione (ISOAC1) against Aβ1-42-induced toxicity. Material and methods: We employed spectroscopic and computational approaches to explore ISOAC1’s ability to mitigate Aβ1-42 aggregation and its consequent toxicity. Other experimental analyses included Th iofl avin T fl uorescence assay, and Western blotting. Results: Our fi ndings reveal ISOAC1’s capacity to disrupt Aβ1-42 aggregation and hinder its transition towards β-sheet structures. Computational investigations elucidate ISOAC1’s binding mechanisms with Aβ1-42, highlighting its potential as a therapeutic agent. Conclusions: ISOAC1 emerges as a promising neuroprotective compound, off ering insights into novel therapeutic strategies for AD treatment. Th e combined spectroscopic and computational approach herein presented provides a comprehensive understanding of ISOAC1’s mechanism of action against Aβ1-42-induced toxicity.