Chemical investigation of secondary metabolites from marine-derived fungus Clonostachys rosea with antiviral and antimicrobial potential

The oceans represent a still largely unexplored environment for the discovery of new bioactive molecules. Due to their biodiversity and extreme environmental conditions, they have provided a basis for the development of a wide range of compounds with significant biological activities. Many bioactive molecules have been discovered, such as alkaloids, terpenoids and peptides, extracted from marine organisms. Marine fungi, especially in recent years, have become a significant subject of interest due to their ability to produce secondary metabolites with unique chemical structures and powerful biological properties [1]. Marine-derived strains of Clonostachys rosea have been found to produce peptaboils, a group of linear peptide antibiotics characterized by non-proteinogenic amino acids [2]. These compounds exhibit strong antifungal and antibacterial activity. Their unique structures and potent bioactivity make them attractive candidates for pharmaceutical development, especially in the search for new antibiotics and antifungal agents. This work is part of a multidisciplinary project, founded by Italian MIUR PRIN 2022, aimed to find new bioactive molecules from extremophilic marine fungi active against emerging or multi-resistant pathogens. This poster shows the extraction, molecular networking, bio guided assay and OSMAC approach used for the identification of a new library of peptaboils from Clonostachys rosea ([Fig. 1]).