Sheep oocytes were collected either by vacuum aspiration with a 20-gauge needle or by slicing ovaries obtained from a local slaughterhouse. The oocytes were in vitro matured, fertilized and cultured as described previously (Thompson et al, Biol. Reprod. 1995, 53:1385-1391). The rate of embryo development was recorded in sheep oocytes matured in IVM medium supplemented with 0, 50, 100, 200 uM cysteamine (exp.1) or with 0, 50, 100, 200 uM l beta-mercaptoethanol (exp.2). After IVM, GSH levels were measured in oocytes matured with 200 pM cysteamine or 50 uM beta-mercaptoethanol with or without 5 mM buthionine sulfoximide (BSO), an inhibitor of GSH synthesis. Logarithmic transformed data were analyzed by ANOVA and Tukey's test. It was demonstrated that 200 uM cysteamine stimulates embryo development (50.2 vs 33.7%) whereas beta-mercaptoethanol does not (31.8 vs 38.1%). Furthermore, GSH synthesis was stimulated in IVM oocytes in the presence of either cysteamine (6.7 vs 4.2 picomol/oocyte) or beta-mercaptoethanol (8.8 vs 6.5 picomol/oocyte). Moreover, the results obtained in the control groups _+ BSO suggests that intracellular GSH synthesis occurs.
Glutathione synthesis during in vitro maturation of ovine oocytes: effect of cysteamine and β-mercaptoethanol / DE MATOS, D. G.; Gasparrini, Bianca; Furnus, C. C.; Thompson, J. G.. - In: THERIOGENOLOGY. - ISSN 0093-691X. - STAMPA. - 51 (1):(1999), pp. 368-368. [10.1016/S0093-691X(99)91927-1]
Glutathione synthesis during in vitro maturation of ovine oocytes: effect of cysteamine and β-mercaptoethanol
GASPARRINI, BIANCA;
1999
Abstract
Sheep oocytes were collected either by vacuum aspiration with a 20-gauge needle or by slicing ovaries obtained from a local slaughterhouse. The oocytes were in vitro matured, fertilized and cultured as described previously (Thompson et al, Biol. Reprod. 1995, 53:1385-1391). The rate of embryo development was recorded in sheep oocytes matured in IVM medium supplemented with 0, 50, 100, 200 uM cysteamine (exp.1) or with 0, 50, 100, 200 uM l beta-mercaptoethanol (exp.2). After IVM, GSH levels were measured in oocytes matured with 200 pM cysteamine or 50 uM beta-mercaptoethanol with or without 5 mM buthionine sulfoximide (BSO), an inhibitor of GSH synthesis. Logarithmic transformed data were analyzed by ANOVA and Tukey's test. It was demonstrated that 200 uM cysteamine stimulates embryo development (50.2 vs 33.7%) whereas beta-mercaptoethanol does not (31.8 vs 38.1%). Furthermore, GSH synthesis was stimulated in IVM oocytes in the presence of either cysteamine (6.7 vs 4.2 picomol/oocyte) or beta-mercaptoethanol (8.8 vs 6.5 picomol/oocyte). Moreover, the results obtained in the control groups _+ BSO suggests that intracellular GSH synthesis occurs.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
