Although several studies have been undertaken on river buffalo chromosomes,the clinical cytogenetics is relatively recent in this important bovid. Indeed,only when speci¢c research programs on females river buffaloes with reproductive disturbances and a collaborative work between cytogeneticists and veterinary practioners were undertaken,significant results will be obtained. In this study we summarize the results of a cytogenetic investigation performed during the last three years on females with reproductive problems and two males free-martin. Peripheral blood cultures were untreated and treated with BrdU and H-33258 six hours before harvesting to get normal and R-banded preparations,respectively. Slides were treated for CBA and R-banding techniques. Some specific cases were also treated for FISH-technique. Upon 59 investigated animals,of which 57 were females with reproductive problems and 2 were males free-martin, 14 (24%) were carriers of sex chromosome abnormalities. The following abnormalities were found in the females: (a) sex reversal syndrome (2n =50,XY) in two animals; (b) X-monosomy (2n =49,X0) in one animal; (c) X-trisomy (2n =51,XXX) in one animal; (d) free-martinism (2n =50,XY/XX with different frequencies of female and male cells among the animals) in 6 females; (e) a pronounced C-band polymorphism in the X-chromosomes of two females. Both rectal palpation in all females and direct observation of some reproductive organs (after the animal’s mating) revealed evident abnormalities in the internal reproductive organs.
Clinical cytogenetics in river buffalo (Bubalus bubalis, 2n = 50): new cases / Iannuzzi, L.; DI MEO, G. P.; Perucatti, A.; DI PALO, Rossella; Zicarelli, Luigi. - In: CHROMOSOME RESEARCH. - ISSN 0967-3849. - STAMPA. - 10 (1):(2002), pp. 27-28.
Clinical cytogenetics in river buffalo (Bubalus bubalis, 2n = 50): new cases.
DI PALO, ROSSELLA;ZICARELLI, LUIGI
2002
Abstract
Although several studies have been undertaken on river buffalo chromosomes,the clinical cytogenetics is relatively recent in this important bovid. Indeed,only when speci¢c research programs on females river buffaloes with reproductive disturbances and a collaborative work between cytogeneticists and veterinary practioners were undertaken,significant results will be obtained. In this study we summarize the results of a cytogenetic investigation performed during the last three years on females with reproductive problems and two males free-martin. Peripheral blood cultures were untreated and treated with BrdU and H-33258 six hours before harvesting to get normal and R-banded preparations,respectively. Slides were treated for CBA and R-banding techniques. Some specific cases were also treated for FISH-technique. Upon 59 investigated animals,of which 57 were females with reproductive problems and 2 were males free-martin, 14 (24%) were carriers of sex chromosome abnormalities. The following abnormalities were found in the females: (a) sex reversal syndrome (2n =50,XY) in two animals; (b) X-monosomy (2n =49,X0) in one animal; (c) X-trisomy (2n =51,XXX) in one animal; (d) free-martinism (2n =50,XY/XX with different frequencies of female and male cells among the animals) in 6 females; (e) a pronounced C-band polymorphism in the X-chromosomes of two females. Both rectal palpation in all females and direct observation of some reproductive organs (after the animal’s mating) revealed evident abnormalities in the internal reproductive organs.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.