Isothermal titration calorimetry (ITC) is a sensitive technique for probing bimolecular processes and can provide direct information about the binding affinity and stoichiometry and the key thermodynamic parameters involved. ITC has been used to investigate the interaction of the ligand H2TMPyP to the two DNA quadruplexes, [d(AGGGT)]4 and [d(TGGGGT)]. Analysis of the ITC data reveals that porphyrin/quadruplex binding stoichiometry under saturating conditions is 1:2 for [d(AGGGT)]4 and 2:1 for [d(TGGGGT)], respectively.
Specific Binding of phorphyrin to G-quadruplex structures / Petraccone, Luigi; Erra, Eva; Esposito, Veronica; Randazzo, Antonio; Mayol, Luciano; J. E., Ladbury; Barone, Guido; Giancola, Concetta. - STAMPA. - (2004), pp. 233-233. (Intervento presentato al convegno XIII International Congress on Thermal Analysis and Calorimetry “XXVI Conference AICAT-GICAT tenutosi a Chia Laguna, Cagliari, Italy. nel 12-19 Settembre 2004).
Specific Binding of phorphyrin to G-quadruplex structures
PETRACCONE, LUIGI;ERRA, EVA;ESPOSITO, VERONICA;RANDAZZO, ANTONIO;MAYOL, LUCIANO;BARONE, GUIDO;GIANCOLA, CONCETTA
2004
Abstract
Isothermal titration calorimetry (ITC) is a sensitive technique for probing bimolecular processes and can provide direct information about the binding affinity and stoichiometry and the key thermodynamic parameters involved. ITC has been used to investigate the interaction of the ligand H2TMPyP to the two DNA quadruplexes, [d(AGGGT)]4 and [d(TGGGGT)]. Analysis of the ITC data reveals that porphyrin/quadruplex binding stoichiometry under saturating conditions is 1:2 for [d(AGGGT)]4 and 2:1 for [d(TGGGGT)], respectively.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
