The aim of this study was to ascertain ovulation rate and non-surgical embryo recovery in buffalo treated with GnRH agonist-LH protocol, and injected with LH 12 or 24 hours after discontinuation of treatment with FSH. Mixed parity buffaloes (n=18) were randomly assigned to three groups: Groups 1 and 2 were implanted with GnRH agonist on Day -14 and injected with estradiol benzoate (10 mg) + progesterone (100 mg) on Day -6. Group 3 received an intravaginal progesterone device (PRID) with estradiol capsule on Day -6. Follicular growth was stimulated with FSH given as equal doses 2x daily in decreasing amounts from Day 0 to Day 4. On day 3 buffaloes were injected with PGF and PRID was removed from Group 3. Groups 1 and 2 were injected with 25 mg LH 12 h (Day 4, P.M.) and 24 h (Day 5, A.M.), respectively, after FSH. Buffaloes were mated by AI and embryo were recovered on Day 5 after AI. The number of follicles were similar among the groups. Group 1 had fewer ovulations and, consequently, corpora lutea compared to the other groups. Also embryo recovery was higher for Groups 2 and 3 compared to Group 1.
Embryo recovery in buffalo with timed ovulation and insemination subsequent to follicle superstimulation / Zicarelli, Luigi; Baruselli, P. S.; Campanile, Giuseppe; DI PALO, Rossella; Gasparrini, Bianca; Neglia, Gianluca; D’Occhio, M. J.. - STAMPA. - 2:(2000), pp. 125-125. (Intervento presentato al convegno 14th International Congress on Animal Reproduction tenutosi a Stockholm, Sweden nel 2-6, July, 2000).
Embryo recovery in buffalo with timed ovulation and insemination subsequent to follicle superstimulation.
ZICARELLI, LUIGI;CAMPANILE, GIUSEPPE;DI PALO, ROSSELLA;GASPARRINI, BIANCA;NEGLIA, GIANLUCA;
2000
Abstract
The aim of this study was to ascertain ovulation rate and non-surgical embryo recovery in buffalo treated with GnRH agonist-LH protocol, and injected with LH 12 or 24 hours after discontinuation of treatment with FSH. Mixed parity buffaloes (n=18) were randomly assigned to three groups: Groups 1 and 2 were implanted with GnRH agonist on Day -14 and injected with estradiol benzoate (10 mg) + progesterone (100 mg) on Day -6. Group 3 received an intravaginal progesterone device (PRID) with estradiol capsule on Day -6. Follicular growth was stimulated with FSH given as equal doses 2x daily in decreasing amounts from Day 0 to Day 4. On day 3 buffaloes were injected with PGF and PRID was removed from Group 3. Groups 1 and 2 were injected with 25 mg LH 12 h (Day 4, P.M.) and 24 h (Day 5, A.M.), respectively, after FSH. Buffaloes were mated by AI and embryo were recovered on Day 5 after AI. The number of follicles were similar among the groups. Group 1 had fewer ovulations and, consequently, corpora lutea compared to the other groups. Also embryo recovery was higher for Groups 2 and 3 compared to Group 1.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
