Yak and cattle sex-chromosome specific DNA probes were used for sexing water buffalo spermatozoa by fluorescence in situ hybridazion (FISH). The X- and Y-specific paint set made from sorted yak chromosomes effectively labeled the respective chromosomes of water buffalo, except the centomeres. Cattle Yp repeat sequences (BC 1.2) showed strong signal on the telomeric region of BBU Yq, representing the evolutionary conservation of this locus in water buffalo for the first time. Both methods might be used to control the separation of X and Y bearing spermatozoa also in this species.
FISH analysis of X- and Y-bearing water buffalo spermatozoa / Revay, T.; Kovacs, A.; Presicce, G. A.; Senatore, E. M.; Neglia, Gianluca; Rens, W.; Gustavsson, I.. - STAMPA. - --:(2002), pp. 40-40. (Intervento presentato al convegno 15th European Colloquium on Cytogenetics of Domestic Animals and Gene Mapping tenutosi a Sorrento, Naples, Italy nel June 2–4, 2002).
FISH analysis of X- and Y-bearing water buffalo spermatozoa.
NEGLIA, GIANLUCA;
2002
Abstract
Yak and cattle sex-chromosome specific DNA probes were used for sexing water buffalo spermatozoa by fluorescence in situ hybridazion (FISH). The X- and Y-specific paint set made from sorted yak chromosomes effectively labeled the respective chromosomes of water buffalo, except the centomeres. Cattle Yp repeat sequences (BC 1.2) showed strong signal on the telomeric region of BBU Yq, representing the evolutionary conservation of this locus in water buffalo for the first time. Both methods might be used to control the separation of X and Y bearing spermatozoa also in this species.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
