The aim of this study was to verify the pituitary response to a GnRH administration on day 6 post-insemination in buffalo. The trial was carried out in January on 21 pluriparous Italian Mediterranean Buffaloes (DIM=118±50 days). The animals were synchronized by using the Ovsynch-TAI Program (Neglia et al., 2003) and inseminated twice, 16 and 40 hours after the last administration of GnRH. Blood samples were collected just before GnRH administration (time 0 – T0) and 30 minutes (time 1 – T1), 3 hours (time 3 – T3) and 24 hours (time 24 – T24) after administration, in order to determine LH, FSH and E2 levels. The administration of 12 μg of Buserelin acetate (Figure 1) significantly (P<0.01) increased LH and FSH blood levels after 30 minutes (T1) and after 3 hours (T3), lowering to the initial values after 24 hours (T24). Therefore the use of GnRH or its analogous represents a good tool in order to increase P4 levels in buffalo species.
Effect of GnRH administration on pituitary response in buffalo / Vecchio, Domenico; Neglia, Gianluca; Comin, A.; Renaville, B.; Campanile, Giuseppe. - STAMPA. - (2005), pp. 259-260. (Intervento presentato al convegno 3° Congresso Nazionale sull’Allevamento del Bufalo e 1° International Symposium of Europe and Americas tenutosi a Capaccio – Paestum, Salerno, Italy nel October 12 – 15, 2005).
Effect of GnRH administration on pituitary response in buffalo.
VECCHIO, DOMENICO;NEGLIA, GIANLUCA;CAMPANILE, GIUSEPPE
2005
Abstract
The aim of this study was to verify the pituitary response to a GnRH administration on day 6 post-insemination in buffalo. The trial was carried out in January on 21 pluriparous Italian Mediterranean Buffaloes (DIM=118±50 days). The animals were synchronized by using the Ovsynch-TAI Program (Neglia et al., 2003) and inseminated twice, 16 and 40 hours after the last administration of GnRH. Blood samples were collected just before GnRH administration (time 0 – T0) and 30 minutes (time 1 – T1), 3 hours (time 3 – T3) and 24 hours (time 24 – T24) after administration, in order to determine LH, FSH and E2 levels. The administration of 12 μg of Buserelin acetate (Figure 1) significantly (P<0.01) increased LH and FSH blood levels after 30 minutes (T1) and after 3 hours (T3), lowering to the initial values after 24 hours (T24). Therefore the use of GnRH or its analogous represents a good tool in order to increase P4 levels in buffalo species.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
