Analysis of the goat beta-lactoglobulin gene evidenced three differences, two in the 7th exon (a G--A transition in position 83 and a 10 nucleotides, 5'-AGGCCCCTCC-3', insertion/deletion rearrangement phenomenon located downstream nucleotide 104) and one in the 6th intron (an A--G transition located 7 nucleotides upstream the 7th exon). In order to identify carriers of the two exonic mutations, a PCR-RFLP method of analysis has been set up. The frequency of the insertion in a local goat population reared in province of Naples and in Girgentana goats reared in Basilicata and Sicilia was 0.09 and 0.01 respectively
Molecular analysis of the goat b-lactoglobulin gene: preliminary results / Pappalardo, Margherita; Gallo, Daniela; Cosenza, Gianfranco; Pastore, N.; Senese, C.; Rubino, R.; Marletta, D.; Ramunno, Luigi. - (2001), pp. 61-63. (Intervento presentato al convegno A.S.P.A.14th Congress tenutosi a Firenze nel 12-15 giugno).
Molecular analysis of the goat b-lactoglobulin gene: preliminary results.
PAPPALARDO, MARGHERITA;GALLO, DANIELA;COSENZA, GIANFRANCO;RAMUNNO, LUIGI
2001
Abstract
Analysis of the goat beta-lactoglobulin gene evidenced three differences, two in the 7th exon (a G--A transition in position 83 and a 10 nucleotides, 5'-AGGCCCCTCC-3', insertion/deletion rearrangement phenomenon located downstream nucleotide 104) and one in the 6th intron (an A--G transition located 7 nucleotides upstream the 7th exon). In order to identify carriers of the two exonic mutations, a PCR-RFLP method of analysis has been set up. The frequency of the insertion in a local goat population reared in province of Naples and in Girgentana goats reared in Basilicata and Sicilia was 0.09 and 0.01 respectivelyI documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
