To unravel the expression of the Shaker-related delayed rectifier potassium subunits Kv1.1 and Kv1.2 in the ileum of three murine strains. Immunofluorescence using cell type markers was performed on cryosections and whole mounts, containing the myenteric or submucous plexus, which were analysed by fluorescence and confocal microscopy. Distribution of Kv1.1 and Kv1.2 was similar in the ileum of the three murine strains. Kv1.1 was found in myenteric ganglionic S100-immunoreactive (ir) glial cells, in almost all extraganglionic S100-ir glial cells, in S100-ir glial cells of the submucosa. Kv1.1 was also detected in some GFAP-ir glial cells of the myenteric ganglia and in the GFAP-ir glial cells of the submucous internodal strands. Kv1.2 was found in intramuscular and submucous S100-ir glial cells, and in a few GFAP-ir glial cells. Kv1.1 or Kv1.2 were not found in interstitial cells of Cajal. Kv1.2 was expressed in some ganglionic nerve cell bodies. A part of these neurons was cholinergic. Kv1.1 and Kv1.2 are predominantly expressed in distinct phenotypes of the ileal enteroglial cells. Kv1.2 is also expressed on the somata of distinct enteric subpopulations. These results support the active role of enteroglial cells in intestinal motility.
Expression of the voltage-gated delayed-rectifier potassium subunits kv1.1 and kv1.2 in the murine intestine / Costagliola, Anna; VAN NASSAUW, L.; Snyders, D.; Adriaensen, D.; Timmermans, J. P.. - ELETTRONICO. - (2009), pp. 103-103. (Intervento presentato al convegno Joint Meeting 2009 Anatomische Gesellschaft –Nederlandse Anatomen Vereniging tenutosi a Antwerpen nel 27-30 marzo) [10.3337/anatges.2009.0009].
Expression of the voltage-gated delayed-rectifier potassium subunits kv1.1 and kv1.2 in the murine intestine.
COSTAGLIOLA, ANNA;
2009
Abstract
To unravel the expression of the Shaker-related delayed rectifier potassium subunits Kv1.1 and Kv1.2 in the ileum of three murine strains. Immunofluorescence using cell type markers was performed on cryosections and whole mounts, containing the myenteric or submucous plexus, which were analysed by fluorescence and confocal microscopy. Distribution of Kv1.1 and Kv1.2 was similar in the ileum of the three murine strains. Kv1.1 was found in myenteric ganglionic S100-immunoreactive (ir) glial cells, in almost all extraganglionic S100-ir glial cells, in S100-ir glial cells of the submucosa. Kv1.1 was also detected in some GFAP-ir glial cells of the myenteric ganglia and in the GFAP-ir glial cells of the submucous internodal strands. Kv1.2 was found in intramuscular and submucous S100-ir glial cells, and in a few GFAP-ir glial cells. Kv1.1 or Kv1.2 were not found in interstitial cells of Cajal. Kv1.2 was expressed in some ganglionic nerve cell bodies. A part of these neurons was cholinergic. Kv1.1 and Kv1.2 are predominantly expressed in distinct phenotypes of the ileal enteroglial cells. Kv1.2 is also expressed on the somata of distinct enteric subpopulations. These results support the active role of enteroglial cells in intestinal motility.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
