Ascorbic acid (AsA) and polyphenols (PP) are secondary metabolites important for plant cell protection against oxidative stresses. In addition, food with high content of those compounds are useful for human health. Among vegetables, tomato is the most important specie due to its significant consumption at worldwide level.; howeverHowever, very little is known about antioxidant genetic regulation in this specie. In order to provide additional biological systems for studying identify changes in the antioxidant gene expression associated to the antioxidant control in tomato we have obtained callus from Solanum lycopersicum cv. M82 and S. pennellii introgression lines (ILs) IL7-3 and IL12-4. ; which fruit IL 12-4 have been previously shown to express a QTL for increased fruit AsA content while IL7-3 showed higher fruit content of AsA and PP is higher than M82. Callus tissue offers the possibility to avoid effects due to the physiological status of the plant that can often masks single gene activity. Callus cultures were successfully obtained from unpeeled pericarp explants of mature green (MG), turning red (TR) and red ripe (RR) fruit. Frequencies of callus, AsA and PP were explants and genotype dependent. High percentage of callus was observed in all explants of screened genotypes except for RR explant of IL12-4 fruits. After 60 days of in vitro culture AsA and PP were found to be higher than the differentiated tissues in all genotypes and explants assayed. In differentiated tissues and calli a decrease ofreduced expression of genes involved in cell wall metabolism and ethylene biosynthesis AsA synthesis, though the cell wall degradation pathway, was observed in both ILs than M82. Moreover, despite the AsA accumulation observed in calli obtained from MG fruits of ILs, than the relative differentiated tissues, a decrease of gene expression was observed. Further gene expression analyses will elucidate genetic mechanisms controlling AsA and PP accumulation in tomato.
Antioxidant synthesis and accumulation in callus culture of tomato fruit / Minutolo, Maria; DI MATTEO, Antonio; Chiaiese, L.; Lotti, E.; Errico, Angela. - STAMPA. - (2010), pp. 132-132. (Intervento presentato al convegno Seventh Solanaceae Conference tenutosi a Dundee, Scottland (UK) nel 5-9 September 2010).
Antioxidant synthesis and accumulation in callus culture of tomato fruit
MINUTOLO, MARIA;DI MATTEO, ANTONIO;ERRICO, ANGELA
2010
Abstract
Ascorbic acid (AsA) and polyphenols (PP) are secondary metabolites important for plant cell protection against oxidative stresses. In addition, food with high content of those compounds are useful for human health. Among vegetables, tomato is the most important specie due to its significant consumption at worldwide level.; howeverHowever, very little is known about antioxidant genetic regulation in this specie. In order to provide additional biological systems for studying identify changes in the antioxidant gene expression associated to the antioxidant control in tomato we have obtained callus from Solanum lycopersicum cv. M82 and S. pennellii introgression lines (ILs) IL7-3 and IL12-4. ; which fruit IL 12-4 have been previously shown to express a QTL for increased fruit AsA content while IL7-3 showed higher fruit content of AsA and PP is higher than M82. Callus tissue offers the possibility to avoid effects due to the physiological status of the plant that can often masks single gene activity. Callus cultures were successfully obtained from unpeeled pericarp explants of mature green (MG), turning red (TR) and red ripe (RR) fruit. Frequencies of callus, AsA and PP were explants and genotype dependent. High percentage of callus was observed in all explants of screened genotypes except for RR explant of IL12-4 fruits. After 60 days of in vitro culture AsA and PP were found to be higher than the differentiated tissues in all genotypes and explants assayed. In differentiated tissues and calli a decrease ofreduced expression of genes involved in cell wall metabolism and ethylene biosynthesis AsA synthesis, though the cell wall degradation pathway, was observed in both ILs than M82. Moreover, despite the AsA accumulation observed in calli obtained from MG fruits of ILs, than the relative differentiated tissues, a decrease of gene expression was observed. Further gene expression analyses will elucidate genetic mechanisms controlling AsA and PP accumulation in tomato.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
