Previous studies of psoriatic epidermis showed that keratinocytes within psoriatic plaques have a resistance to apoptosis and are characterized by increased cell proliferation if compared with normal skin. The resistance to apoptosis has been particularly linked to the absence of a significant amount of double-stranded DNA breaks, in spite of the reported TUNEL (Terminal deoxynucleotidyl Transferase Biotin-dUTP Nick End Labeling) reactivity of most psoriatic lesions. This phenomenon has been interpreted as due to the increase in the number of DNA nicks resulting from active DNA replication, instead that DNA double-strands breaks resulting from the apoptotic process. CAF-1 p60 is a newly proposed proliferation marker, which plays a key role in chromatin assembly during cell replication and DNA repair. It is a part of a heterotrimeric complex (CAF-1) that regulates, in particular, DNA chromosome assembly during cell replication and the resulting chromatin structure after nucleotide excision repair (NER)-mediated DNA repair. CAF-1 has to interact with PCNA (Proliferating Cell Nuclear Antigen); p60, in particular, is down-regulated in quiescent cells, whereas it is over-expressed in hyperproliferating and neoplastic cells. We analyzed the immunohistochemical expression of p60 and PCNA in a series of plaque psoriasis samples (formalin-fixed, paraffin-embedded). A significant higher labelling of psoriatic keratinocytes for p60 than to PCNA was found. Our data suggest that the immunohistochemical p60 expression could represent a sensible instrument to evaluate the deregulation of cell proliferation in psoriasis, giving useful informations on both the cell-cycle progression and DNA-repair status of keratinocytes. In addition p60 expression could represent a valuable tool to evaluate the effect of therapy in follow-up of psoriatic patients.
CAF-1 p60 as a new marker of cell proliferation in plaque psoriasis / Staibano, Stefania; A., Longobardi; G., Salvatore; M., Di Benedetto; Mascolo, Massimo; M., Nino; N., Balato; DE ROSA, Gaetano; Ayala, Fabio. - In: JOURNAL OF INVESTIGATIVE DERMATOLOGY. - ISSN 0022-202X. - STAMPA. - (2007), pp. 71-71. (Intervento presentato al convegno 37th Annual Meeting of the European Society of Dermatology Research tenutosi a Zurich nel 5-8 September 2007).
CAF-1 p60 as a new marker of cell proliferation in plaque psoriasis
STAIBANO, STEFANIA;MASCOLO, MASSIMO;DE ROSA, GAETANO;AYALA, FABIO
2007
Abstract
Previous studies of psoriatic epidermis showed that keratinocytes within psoriatic plaques have a resistance to apoptosis and are characterized by increased cell proliferation if compared with normal skin. The resistance to apoptosis has been particularly linked to the absence of a significant amount of double-stranded DNA breaks, in spite of the reported TUNEL (Terminal deoxynucleotidyl Transferase Biotin-dUTP Nick End Labeling) reactivity of most psoriatic lesions. This phenomenon has been interpreted as due to the increase in the number of DNA nicks resulting from active DNA replication, instead that DNA double-strands breaks resulting from the apoptotic process. CAF-1 p60 is a newly proposed proliferation marker, which plays a key role in chromatin assembly during cell replication and DNA repair. It is a part of a heterotrimeric complex (CAF-1) that regulates, in particular, DNA chromosome assembly during cell replication and the resulting chromatin structure after nucleotide excision repair (NER)-mediated DNA repair. CAF-1 has to interact with PCNA (Proliferating Cell Nuclear Antigen); p60, in particular, is down-regulated in quiescent cells, whereas it is over-expressed in hyperproliferating and neoplastic cells. We analyzed the immunohistochemical expression of p60 and PCNA in a series of plaque psoriasis samples (formalin-fixed, paraffin-embedded). A significant higher labelling of psoriatic keratinocytes for p60 than to PCNA was found. Our data suggest that the immunohistochemical p60 expression could represent a sensible instrument to evaluate the deregulation of cell proliferation in psoriasis, giving useful informations on both the cell-cycle progression and DNA-repair status of keratinocytes. In addition p60 expression could represent a valuable tool to evaluate the effect of therapy in follow-up of psoriatic patients.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
