In this work, we have fabricated a porous silicon (PSi) Bragg reflectors microarray using a proper technological process based on photolithography and electrochemical anodization of silicon. Each element of the array is characterized by a diameter of 200 μm. The PSi structures have been used as platform to immobilize label-free DNA probe and a simple optical method has been employed to investigate the interaction between probe-DNA and its complementary target. In order to confirm the specificity of the DNA hybridization, we have also verified that the reaction of probe-DNA with non-complementary DNA did not occur.
A porous silicon based microarray for label-free optical detection of DNA hybridization / I., Rea; G., Coppola; M., Gioffrè; E., De Tommasi; I., Rendina; Lamberti, Annalisa; L., De Stefano. - 7606:(2010), pp. 76060E-1-76060E-5. (Intervento presentato al convegno Silicon Photonics V tenutosi a San Francisco, California nel 23 gennaio 2010) [10.1117/12.848471].
A porous silicon based microarray for label-free optical detection of DNA hybridization
LAMBERTI, ANNALISA;
2010
Abstract
In this work, we have fabricated a porous silicon (PSi) Bragg reflectors microarray using a proper technological process based on photolithography and electrochemical anodization of silicon. Each element of the array is characterized by a diameter of 200 μm. The PSi structures have been used as platform to immobilize label-free DNA probe and a simple optical method has been employed to investigate the interaction between probe-DNA and its complementary target. In order to confirm the specificity of the DNA hybridization, we have also verified that the reaction of probe-DNA with non-complementary DNA did not occur.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
