A series of known G-quadruplex ligands have been tested by ESI-MS spectrometry with (TG(4)T)(4) and analogues I and II blocked respectively at the 3' or 5'-end by a tetra-end-linked (TEL) unit. The stoichiometries of these complexes and on ligand. affinity and selectivity to the different quadruplexes were determined to deduce the ligand binding site. The TEL derivatives I and II allow probing the contribution of groove binding to the G-quadruplex, and demonstrating that 3' and 5' quartets are, not equivalent binding sites for ligand end-stacking.
Ligand binding to tetra-end-linked (TGGGGT)4 G-quadruplexes: an electrospray mass spectrometry study / Oliviero, Giorgia; Amato, Jussara; D'Errico, Stefano; Piccialli, Gennaro; Mailliet, P; Rosu, F; de Pauw, E; Gabelica, V.. - STAMPA. - 10:(2008), pp. 333-335. (Intervento presentato al convegno XIV symposium on chemistry of nucleic acid components tenutosi a Cesky Krumlov nel 8-13 Giugno 2008).
Ligand binding to tetra-end-linked (TGGGGT)4 G-quadruplexes: an electrospray mass spectrometry study.
OLIVIERO, GIORGIA;AMATO, JUSSARA;D'ERRICO, STEFANO;PICCIALLI, GENNARO;
2008
Abstract
A series of known G-quadruplex ligands have been tested by ESI-MS spectrometry with (TG(4)T)(4) and analogues I and II blocked respectively at the 3' or 5'-end by a tetra-end-linked (TEL) unit. The stoichiometries of these complexes and on ligand. affinity and selectivity to the different quadruplexes were determined to deduce the ligand binding site. The TEL derivatives I and II allow probing the contribution of groove binding to the G-quadruplex, and demonstrating that 3' and 5' quartets are, not equivalent binding sites for ligand end-stacking.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
