The search for new materials and innovative scaffolds to improve cell adhesion and accelerate tissue regeneration for applications in wound healing and bone repair has received increasing interest.1 We report the synthesis and characterization of biodegradable, bioresorbable scaffolds based on polylactic acid (PLA) that can act as temporary extracellular matrix and bear surface modification with polyphenols suitable to promote a general amelioration of the healing processes. Natural phenolic antioxidants including resveratrol2 and green tea catechins3 have been recently investigated in tissue engineering as promoters of osteoblast differentiations. Polyphenol polymers, namely polytyrosol or polyCAME, 4 were prepared in good yields by biomimetic oxidation of naturally occurring compounds like tyrosol and caffeic acid ester, were subjected to structural characterization and shown to possess an antioxidant action of potential relevance to tissue healing. Expected advantages with respect to the monomers include greater chemical stability and lower tendency to be released from the scaffold into the contact medium. PLA sponge-like 3D-scaffolds were obtained by freeze drying of tetrahydrofuran solution of PLA/polyphenol and the extent of polyphenol incorporation (3 to 10%) was determined by spectrophotometric/ HPLC analyses. The composites show no toxicity toward human osteosarcoma Saos-2 cells, enhancement of ALP activity and packing efficiency of the osteoblasts compared to the scaffold not containing the polyphenols
POLYPHENOL MODIFIED POLYLACTIC ACID SPONGES WITH IMPROVED OSTEOGENIC PROPERTIES / S., Antenucci; H., Farina; M., Ortenzi; Panzella, Lucia; Napolitano, Alessandra; B., Burlando; E., Ranzato; S., Martinotti; L., Verotta. - (2015), pp. 47-47. (Intervento presentato al convegno 9th World Congress on Polyphenols Applications tenutosi a Malta nel 3-5/6/2015).
POLYPHENOL MODIFIED POLYLACTIC ACID SPONGES WITH IMPROVED OSTEOGENIC PROPERTIES
PANZELLA, LUCIA;NAPOLITANO, ALESSANDRA;
2015
Abstract
The search for new materials and innovative scaffolds to improve cell adhesion and accelerate tissue regeneration for applications in wound healing and bone repair has received increasing interest.1 We report the synthesis and characterization of biodegradable, bioresorbable scaffolds based on polylactic acid (PLA) that can act as temporary extracellular matrix and bear surface modification with polyphenols suitable to promote a general amelioration of the healing processes. Natural phenolic antioxidants including resveratrol2 and green tea catechins3 have been recently investigated in tissue engineering as promoters of osteoblast differentiations. Polyphenol polymers, namely polytyrosol or polyCAME, 4 were prepared in good yields by biomimetic oxidation of naturally occurring compounds like tyrosol and caffeic acid ester, were subjected to structural characterization and shown to possess an antioxidant action of potential relevance to tissue healing. Expected advantages with respect to the monomers include greater chemical stability and lower tendency to be released from the scaffold into the contact medium. PLA sponge-like 3D-scaffolds were obtained by freeze drying of tetrahydrofuran solution of PLA/polyphenol and the extent of polyphenol incorporation (3 to 10%) was determined by spectrophotometric/ HPLC analyses. The composites show no toxicity toward human osteosarcoma Saos-2 cells, enhancement of ALP activity and packing efficiency of the osteoblasts compared to the scaffold not containing the polyphenolsI documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
