Raman microspectroscopy proved to be a valuable support to protein crystallography in all the steps of 3D structure determination: to study changes in chemical composition [1, 2], in secondary [3] and tertiary structure studies [4, 5], and to follow chemical mechanisms [7-9]. Several off-line examples of metal-ion/protein complexes will be reported: a) Ru-based drugs [7-8], b) inhibitors of Zn-binding proteins [5], c) Ir-based CO-releasing molecules [9]. On-line Raman assistance (at SLS synchrotron) will be also presented, in which Raman microscopy clearly reveals X-ray induced-photodamage: d) NO-photodissociation from hemoglobin [2] and e) extensive de-methylation in methionine and de carboxylation of Asp/Glu residues (unpublished results). Perspectives of future SERS (Surface Enhanced Raman Spectroscopy) from single crystals will be introduced for protein-gold nanoparticle co-crystallization studies [10]. [1] A. Vergara, et al., Acta Cryst. Sect D: Biol Crystallogr. (2008) 64, 167-171. [2] A. Merlino, et al. Vergara, A., Acta Cryst. Sect D: Biol Crystallogr. (2013) 69, 137-40. [3] A. Merlino, et al. A. Vergara, Biophys. Chem. (2008) 137, 24-27. [4] A. Vergara, et al., J. Biol. Chem. (2010) 285, 32568-32575. [5] A. Di Fiore, A. Vergara, et al. Chem. Comm. (2015) 51 11519-11522 [7] A. Vergara, et al. Inorg Chem. (2013) 52, 4157-4159. [8] A. Vergara, et al. Inorg Chem (2013) 52, 10714-10716. [9] A. Petruk, A. Vergara et al. Inorg. Chem. (2014), 53(19), 10456–10462. [10] A. Merlino, A. et al. A. Vergara, Nature Nanotech. (2015) 10, 285.
INTERACTION BETWEEN METAL IONS OR NANOPARTICLES WITH PROTEINS. A RAMAN-ASSISTED CRYSTALLOGRAPHY APPROACH / Vergara, Alessandro. - (2016). (Intervento presentato al convegno MISCA 2016. IV MEETING OF THE ITALIAN AND SPANISH CRYSTALLOGRAPHIC ASSOCIATIONS).
INTERACTION BETWEEN METAL IONS OR NANOPARTICLES WITH PROTEINS. A RAMAN-ASSISTED CRYSTALLOGRAPHY APPROACH
VERGARA, ALESSANDRO
2016
Abstract
Raman microspectroscopy proved to be a valuable support to protein crystallography in all the steps of 3D structure determination: to study changes in chemical composition [1, 2], in secondary [3] and tertiary structure studies [4, 5], and to follow chemical mechanisms [7-9]. Several off-line examples of metal-ion/protein complexes will be reported: a) Ru-based drugs [7-8], b) inhibitors of Zn-binding proteins [5], c) Ir-based CO-releasing molecules [9]. On-line Raman assistance (at SLS synchrotron) will be also presented, in which Raman microscopy clearly reveals X-ray induced-photodamage: d) NO-photodissociation from hemoglobin [2] and e) extensive de-methylation in methionine and de carboxylation of Asp/Glu residues (unpublished results). Perspectives of future SERS (Surface Enhanced Raman Spectroscopy) from single crystals will be introduced for protein-gold nanoparticle co-crystallization studies [10]. [1] A. Vergara, et al., Acta Cryst. Sect D: Biol Crystallogr. (2008) 64, 167-171. [2] A. Merlino, et al. Vergara, A., Acta Cryst. Sect D: Biol Crystallogr. (2013) 69, 137-40. [3] A. Merlino, et al. A. Vergara, Biophys. Chem. (2008) 137, 24-27. [4] A. Vergara, et al., J. Biol. Chem. (2010) 285, 32568-32575. [5] A. Di Fiore, A. Vergara, et al. Chem. Comm. (2015) 51 11519-11522 [7] A. Vergara, et al. Inorg Chem. (2013) 52, 4157-4159. [8] A. Vergara, et al. Inorg Chem (2013) 52, 10714-10716. [9] A. Petruk, A. Vergara et al. Inorg. Chem. (2014), 53(19), 10456–10462. [10] A. Merlino, A. et al. A. Vergara, Nature Nanotech. (2015) 10, 285.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
