The characterization of cytoskeleton properties could be a very important diagnostic tool with which to better understand the tumourigenic mechanisms. Here we have consolidated our previous findings as to how high energy X-ray - which are ordinarily employed in radiotherapy - can modify the structure and the activity of the cell cytoskeleton. We studied the effects over time (24 and 72 h) of two different doses of X-rays (1 and 2 Gray) on proliferation, spreading, focal adhesions, migration and mechanical properties of BALB/c3T3 and SV40 transformed BALB/c3T3 cells. We observed that X-rays induced a sensitive increase of transformed cell adhesion, responsible of the decrease of migratory ability and the enhancement of the mechanical properties of SVT2 cells. On the contrary, the effects of X-rays on BALB/c3T3 properties were negligible.
Effects of high energy X-rays on cell morphology and functions / Panzetta, Valeria; Musellav, I.; Pugliese, Mariagabriella; Piccolo, C.; Pasqua, G.; Netti, PAOLO ANTONIO; Fusco, S.. - In: IEEE Xplore Digital Library. - (2017), pp. 1-4. (Intervento presentato al convegno 5th Portuguese Meeting on Bioengineering, ENBENG 2017 tenutosi a Coimbra, Portugal nel 16 - 18 febbraio 2017) [10.1109/ENBENG.2017.7889448].
Effects of high energy X-rays on cell morphology and functions
PANZETTA, VALERIA;PUGLIESE, MARIAGABRIELLA;NETTI, PAOLO ANTONIO;
2017
Abstract
The characterization of cytoskeleton properties could be a very important diagnostic tool with which to better understand the tumourigenic mechanisms. Here we have consolidated our previous findings as to how high energy X-ray - which are ordinarily employed in radiotherapy - can modify the structure and the activity of the cell cytoskeleton. We studied the effects over time (24 and 72 h) of two different doses of X-rays (1 and 2 Gray) on proliferation, spreading, focal adhesions, migration and mechanical properties of BALB/c3T3 and SV40 transformed BALB/c3T3 cells. We observed that X-rays induced a sensitive increase of transformed cell adhesion, responsible of the decrease of migratory ability and the enhancement of the mechanical properties of SVT2 cells. On the contrary, the effects of X-rays on BALB/c3T3 properties were negligible.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
