Most of the recently developed anticancer drugs induce apoptotic cell death in tumor cells through up-regulating the intracellular ROS levels. New evidence suggests the promising role of curcumin, a yellow-gold color phytochemical turmeric, isolated from root of the Curcuma longa, and of graviola, (Annona muricata), a tropical plant belonging to family Annonaceae, known for its medicinal uses, in the treatment of cancer1-2. In our study we analyzed the effects on proliferation and apoptosis in ALL and Jurkat cell line of graviola and curcumin, alone and in combination with various chemotherapeutic agents (Daunorubicin, L-Asparaginase, Metotrexate, Vincristine and Desametazone). The proliferation, apoptosis, cell cycle and ROS production, before and after treatment with a ROS inhibitor, were investigated. Cell fragmentation was observed in Time lapse Imaging. Results: Our preliminary data showed an inhibition of proliferation and an apoptosis induction after 20µg/mL both of curcumin and graviola treatment for 24h. The combined treatment of curcumin respectively with Daunorubicin, L-ASPA, Vincristine and Desametazone showed a significant shift from early to late apoptosis after 24h, using the lowest effective concentration of drugs, compared to the higher dose of drugs alone: the average apoptotic increase was 49 ± 6.3% (p<0.05). Confocal analysis confirmed the internalization of curcumin in Jurkat cells, leading to cytoplasmic and partly nuclear fragmentation, especially when combined with vincristine. Curcumin treatment increased intracellular ROS levels, thus inducing apoptosis in leukemia cells. This selective activity could be attributed to the different redox states between healthy cells and leukemic cells. Curcumin has been described as an inducer of apoptosis and cell cycle arrest via regulating multiple cancer signaling pathways. The molecular insight onto curcumin-mediated anticancer property in leukemia suppression remains to be elucidated. 1.Larasati YA et al. Sci Rep 2018, 8(1):2039 2.Deep G et al. Sci Rep 2016,6:23135
Effects of natural compounds on the oxidative balance in pediatric acute lymphoblastic leukemia / Manca, Rosa; Iannotta, A.; D’Angelo, V.; Di Massa, L.; Di Martino, M.; Pota, E.; Di Pinto, D.; Casale, F.; Pica, Alessandra. - In: EUROPEAN JOURNAL OF HISTOCHEMISTRY. - ISSN 1121-760X. - 62:1(2018), pp. 3-3. [10.4081/ejh.2019.2951]
Effects of natural compounds on the oxidative balance in pediatric acute lymphoblastic leukemia.
Rosa Manca;Alessandra Pica
2018
Abstract
Most of the recently developed anticancer drugs induce apoptotic cell death in tumor cells through up-regulating the intracellular ROS levels. New evidence suggests the promising role of curcumin, a yellow-gold color phytochemical turmeric, isolated from root of the Curcuma longa, and of graviola, (Annona muricata), a tropical plant belonging to family Annonaceae, known for its medicinal uses, in the treatment of cancer1-2. In our study we analyzed the effects on proliferation and apoptosis in ALL and Jurkat cell line of graviola and curcumin, alone and in combination with various chemotherapeutic agents (Daunorubicin, L-Asparaginase, Metotrexate, Vincristine and Desametazone). The proliferation, apoptosis, cell cycle and ROS production, before and after treatment with a ROS inhibitor, were investigated. Cell fragmentation was observed in Time lapse Imaging. Results: Our preliminary data showed an inhibition of proliferation and an apoptosis induction after 20µg/mL both of curcumin and graviola treatment for 24h. The combined treatment of curcumin respectively with Daunorubicin, L-ASPA, Vincristine and Desametazone showed a significant shift from early to late apoptosis after 24h, using the lowest effective concentration of drugs, compared to the higher dose of drugs alone: the average apoptotic increase was 49 ± 6.3% (p<0.05). Confocal analysis confirmed the internalization of curcumin in Jurkat cells, leading to cytoplasmic and partly nuclear fragmentation, especially when combined with vincristine. Curcumin treatment increased intracellular ROS levels, thus inducing apoptosis in leukemia cells. This selective activity could be attributed to the different redox states between healthy cells and leukemic cells. Curcumin has been described as an inducer of apoptosis and cell cycle arrest via regulating multiple cancer signaling pathways. The molecular insight onto curcumin-mediated anticancer property in leukemia suppression remains to be elucidated. 1.Larasati YA et al. Sci Rep 2018, 8(1):2039 2.Deep G et al. Sci Rep 2016,6:23135I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
