In this work, we employed the Raman microscopy to study the internalization kinetics and spatial distribution of small interfering RNA (siRNA)-diatomite nanoparticles (DNPs) complex in human lung epidermoid carcinoma cell line (H1355) up to 72 h. Raman images are compared with confocal fluorescence microscopy results. The Raman analysis provides that the siRNA-DNPs are internalized and co-localized in lipid vesicles within 18 h, after that equilibrium is achieved.

Diatomite nanovectors uptake in cancer cells: A Raman imaging study / Manago, S.; Migliaccio, N.; Terracciano, M.; Napolitano, M.; Martucci, N. M.; De Stefano, L.; Rendina, I.; Lamberti, A.; Rea, I.; De Luca, A. C.. - 10685:(2018), p. 164. (Intervento presentato al convegno Biophotonics: Photonic Solutions for Better Health Care VI 2018 tenutosi a fra nel 2018) [10.1117/12.2315371].

Diatomite nanovectors uptake in cancer cells: A Raman imaging study

Terracciano M.;Martucci N. M.;Lamberti A.;
2018

Abstract

In this work, we employed the Raman microscopy to study the internalization kinetics and spatial distribution of small interfering RNA (siRNA)-diatomite nanoparticles (DNPs) complex in human lung epidermoid carcinoma cell line (H1355) up to 72 h. Raman images are compared with confocal fluorescence microscopy results. The Raman analysis provides that the siRNA-DNPs are internalized and co-localized in lipid vesicles within 18 h, after that equilibrium is achieved.
2018
9781510618961
Diatomite nanovectors uptake in cancer cells: A Raman imaging study / Manago, S.; Migliaccio, N.; Terracciano, M.; Napolitano, M.; Martucci, N. M.; De Stefano, L.; Rendina, I.; Lamberti, A.; Rea, I.; De Luca, A. C.. - 10685:(2018), p. 164. (Intervento presentato al convegno Biophotonics: Photonic Solutions for Better Health Care VI 2018 tenutosi a fra nel 2018) [10.1117/12.2315371].
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11588/793479
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