Mammary tumors are the second most common neoplasia in dogs; therefore, they represent a significant clinical problem, furthermore, recent studies indicate that spontaneous canine mammary carcinomas (CMCs) resemble human breast cancer by clinic and pathology as well as its behavior and prognostic indicators [1, 2] Biological aging pathways may be a mechanism for cognitive impairment in cancer survivors. The goal of the current study was to examine whether indicators of biological aging, namely elevated levels of DNA damage, reduced telomerase enzymatic activity, and shorter peripheral blood telomere length (TL) would be related to cognitive function in a cohort of survivors of breast cancer. [3, 4]. The association of TL with breast cancer prognosis in human had been examined through a systematic review [3]. Our study had the aim to relate peripheral blood TL and canine mammary carcinomas (CMCs) in the bitch. To this end, six female bitches aged 6-8 years old, of middle size, that were submitted to mastectomy for CMC during the last 24 months, were subjected to blood sampling to evaluate TL. As control, 5 clinically healthy bitches free of cancer diseases, and of the same range of age and size were subjected to the same blood withdrawal. From each animal, 8 ml of blood was collected and taken in Qiagen tubes and sent via airmail to the lab of Dana Farber/Harvard Cancer Center Genotyping and Genetics for Population Sciences. DNA extraction was performed on blood white cells, and TL was measured by a modified quantitative polymerase chain reaction qPCR method, as previously described [4]. Our results were submitted to ANOVA test and show that breast cancer patients had significantly shorter TLs than control subjects (p<0.05). The results of the current study suggest a significant association between measures of biological aging and breast cancer. Future prospective studies are needed to confirm a causal role of biological aging as a driver of declines in cognitive function after cancer treatment.
TELOMERE LENGTH AND CANINE MAMMARY CARCINOMAS PROGNOSIS: PRELIMINARY STUDY / Palumbo, Veronica; Ciani, Francesca; Tafuri, Simona; Immaculata De Vivo, ; DEL PRETE, Chiara; Cocchia, Natascia. - (2019), pp. 233-233. (Intervento presentato al convegno Convegno SISVet 2019 tenutosi a Olbia nel 19-22 Giugno 2019).
TELOMERE LENGTH AND CANINE MAMMARY CARCINOMAS PROGNOSIS: PRELIMINARY STUDY
Palumbo Veronica;Francesca Ciani;Simona Tafuri;Del Prete Chiara;Natascia Cocchia
2019
Abstract
Mammary tumors are the second most common neoplasia in dogs; therefore, they represent a significant clinical problem, furthermore, recent studies indicate that spontaneous canine mammary carcinomas (CMCs) resemble human breast cancer by clinic and pathology as well as its behavior and prognostic indicators [1, 2] Biological aging pathways may be a mechanism for cognitive impairment in cancer survivors. The goal of the current study was to examine whether indicators of biological aging, namely elevated levels of DNA damage, reduced telomerase enzymatic activity, and shorter peripheral blood telomere length (TL) would be related to cognitive function in a cohort of survivors of breast cancer. [3, 4]. The association of TL with breast cancer prognosis in human had been examined through a systematic review [3]. Our study had the aim to relate peripheral blood TL and canine mammary carcinomas (CMCs) in the bitch. To this end, six female bitches aged 6-8 years old, of middle size, that were submitted to mastectomy for CMC during the last 24 months, were subjected to blood sampling to evaluate TL. As control, 5 clinically healthy bitches free of cancer diseases, and of the same range of age and size were subjected to the same blood withdrawal. From each animal, 8 ml of blood was collected and taken in Qiagen tubes and sent via airmail to the lab of Dana Farber/Harvard Cancer Center Genotyping and Genetics for Population Sciences. DNA extraction was performed on blood white cells, and TL was measured by a modified quantitative polymerase chain reaction qPCR method, as previously described [4]. Our results were submitted to ANOVA test and show that breast cancer patients had significantly shorter TLs than control subjects (p<0.05). The results of the current study suggest a significant association between measures of biological aging and breast cancer. Future prospective studies are needed to confirm a causal role of biological aging as a driver of declines in cognitive function after cancer treatment.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
