Microgels represent a versatile sub-class of hydrogels enabling new functions in biomolecule detection thanks to the highly tuneable chemistry. The microgels-based platform is composed of multifunctional particles which possess multiple components and properties to fulfil the requirements of the biodetection applications. Indeed, from the synthesis to the final read-out microgels prove advantageous and unique in overcoming issues in detection and quantification directly in biofluids. The chemical flexibility of hydrogel microparticles and microgels allows to embed several building blocks into their structure during the synthesis. Anchoring groups on particles are used to immobilize probes of diverse natures such as antibodies, enzymes, or oligonucleotide strands. In such a way particles active for the detection of a target or more than one biomarker can be obtained. Moreover, the high biocompatibility and antifouling properties of hydrogels make possible to use microgels in biosensing directly in biofluids overcoming washing and separation steps. As for the read-out steps, microgels can be easily manipulated in flow with simple microfluidics making the detection more efficient and sensitive in multiplex context. Here we are presenting a PEG-microgel platform for the detection of oligonucleotides and other biomarkers by using barcoded particles for multiplex analysis. In the final part of the presentation, we discuss on the use of easy microfluidics for the interrogation of microgels in-flow.
Microgels as a Biosensing Platform / Battista, E.; Napoletano, S.; Maremonti, M. I.; Dannhauser, D.; Netti, P. A.; Causa, F.. - (2023). (Intervento presentato al convegno 8th National Congress of Bioengineering, GNB 2023 tenutosi a ita nel 2023).
Microgels as a Biosensing Platform
Napoletano S.;Maremonti M. I.;Dannhauser D.;Netti P. A.;
2023
Abstract
Microgels represent a versatile sub-class of hydrogels enabling new functions in biomolecule detection thanks to the highly tuneable chemistry. The microgels-based platform is composed of multifunctional particles which possess multiple components and properties to fulfil the requirements of the biodetection applications. Indeed, from the synthesis to the final read-out microgels prove advantageous and unique in overcoming issues in detection and quantification directly in biofluids. The chemical flexibility of hydrogel microparticles and microgels allows to embed several building blocks into their structure during the synthesis. Anchoring groups on particles are used to immobilize probes of diverse natures such as antibodies, enzymes, or oligonucleotide strands. In such a way particles active for the detection of a target or more than one biomarker can be obtained. Moreover, the high biocompatibility and antifouling properties of hydrogels make possible to use microgels in biosensing directly in biofluids overcoming washing and separation steps. As for the read-out steps, microgels can be easily manipulated in flow with simple microfluidics making the detection more efficient and sensitive in multiplex context. Here we are presenting a PEG-microgel platform for the detection of oligonucleotides and other biomarkers by using barcoded particles for multiplex analysis. In the final part of the presentation, we discuss on the use of easy microfluidics for the interrogation of microgels in-flow.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
