The presence of microgravity and ionizing radiation during spaceflight missions causes excessive reactive oxygen species (ROS) production that contributes to oxidative cellular stress and multifunctional damage in astronauts. This knowledge has underlined the importance of frequent monitoring of astronaut’s health to have early diagnoses. In this scenario, the biosensor diagnostic devices could offer the necessary analytical performance to study pathological astronaut conditions. Herein, we propose an innovative biosensor for detecting highly diluted biomarkers at picogram level by using the pyro-electrohydrodynamic jet (p-jet) system. The detection limit of the system was confirmed using a model protein as the bovine serum albumin (BSA) by optimizing its deposition on different functionalized glass substrates through different chemical reactions starting with a manual procedure. Based on these results, the epoxy glass activated surface was chosen as the best slide for p-jet experiments. The characterization of the processes was performed through different spectroscopic techniques such as infrared-spectroscopy (IR) or confocal fluorescence. In the context of long-term human missions, our revolutionary approach could be extremely useful to monitor the astronaut health.
Protein immobilization in accumulated pyro-electrohydrodynamic droplets for highly sensitive detection of astronaut health biomarkers / Di Natale, C.; Coppola, S.; Vespini, V.; Tkachenko, V.; Luciani, G.; Vitiello, G.; Ferranti, F.; Mari, S.; Maffettone, P. L.; Grilli, S.. - 12622:(2023). (Intervento presentato al convegno Optical Methods for Inspection, Characterization, and Imaging of Biomaterials VI 2023 tenutosi a deu nel 2023) [10.1117/12.2675813].
Protein immobilization in accumulated pyro-electrohydrodynamic droplets for highly sensitive detection of astronaut health biomarkers
Di Natale C.;Luciani G.;Vitiello G.;Mari S.;Maffettone P. L.;
2023
Abstract
The presence of microgravity and ionizing radiation during spaceflight missions causes excessive reactive oxygen species (ROS) production that contributes to oxidative cellular stress and multifunctional damage in astronauts. This knowledge has underlined the importance of frequent monitoring of astronaut’s health to have early diagnoses. In this scenario, the biosensor diagnostic devices could offer the necessary analytical performance to study pathological astronaut conditions. Herein, we propose an innovative biosensor for detecting highly diluted biomarkers at picogram level by using the pyro-electrohydrodynamic jet (p-jet) system. The detection limit of the system was confirmed using a model protein as the bovine serum albumin (BSA) by optimizing its deposition on different functionalized glass substrates through different chemical reactions starting with a manual procedure. Based on these results, the epoxy glass activated surface was chosen as the best slide for p-jet experiments. The characterization of the processes was performed through different spectroscopic techniques such as infrared-spectroscopy (IR) or confocal fluorescence. In the context of long-term human missions, our revolutionary approach could be extremely useful to monitor the astronaut health.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
