Synthetic and Natural Lipid Bilayer (LB) systems are attracting particular interest due to their distinctive pharmacokinetic profiles and physico-chemical properties. The elastic adaptability owing to the fluidity of lipidic membranes opens exciting possibilities to engineering these structures by properly stimulating them. However, a lack of knowledge of the mechanical responses and the high sensitivity to small perturbations are still hindering the accurate manipulation of these complex surfaces. Microfluidic approaches seem to be a good choice to finely manipulate local environmental conditions, in such a way to precisely alter biomembranes and control the final deformative outcomes. However, to the best of our knowledge, microfluidic approaches are mostly geared at stimulating single vesicles, rather than concentrated suspensions. In a recent work, our group patented an innovative method for the encapsulation of U87-derived exosomes with various molecules, based on the application of high hydrodynamic pressures. It seemed that the pool of forces generated from this stimulation temporarily modified lipidic membranes, allowing the transit of molecules inside exosomes, with the resulting rise of their encapsulation efficiency. Inspired from these results, we propose the application of controlled stresses on population of lipid-based systems as a way of handling, identifying, and manipulating membrane's mechanics and dynamics, in order to improve and widen their industrial applicability.
Engineering of biological entities through dynamic perturbations of lipidic membranes / Silvestri, S.; Romano, E.; Netti, P. A.; Torino, E.. - (2023). (Intervento presentato al convegno 8th National Congress of Bioengineering, GNB 2023 tenutosi a ita nel 2023).
Engineering of biological entities through dynamic perturbations of lipidic membranes
Silvestri S.;Romano E.;Netti P. A.;Torino E.
2023
Abstract
Synthetic and Natural Lipid Bilayer (LB) systems are attracting particular interest due to their distinctive pharmacokinetic profiles and physico-chemical properties. The elastic adaptability owing to the fluidity of lipidic membranes opens exciting possibilities to engineering these structures by properly stimulating them. However, a lack of knowledge of the mechanical responses and the high sensitivity to small perturbations are still hindering the accurate manipulation of these complex surfaces. Microfluidic approaches seem to be a good choice to finely manipulate local environmental conditions, in such a way to precisely alter biomembranes and control the final deformative outcomes. However, to the best of our knowledge, microfluidic approaches are mostly geared at stimulating single vesicles, rather than concentrated suspensions. In a recent work, our group patented an innovative method for the encapsulation of U87-derived exosomes with various molecules, based on the application of high hydrodynamic pressures. It seemed that the pool of forces generated from this stimulation temporarily modified lipidic membranes, allowing the transit of molecules inside exosomes, with the resulting rise of their encapsulation efficiency. Inspired from these results, we propose the application of controlled stresses on population of lipid-based systems as a way of handling, identifying, and manipulating membrane's mechanics and dynamics, in order to improve and widen their industrial applicability.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
