This work studies the binding properties of distamycin and its carbamoyl analog, containing four pyrrole units, with the [d(TGGGGT)](4) quadruplex by means of isothermal titration calorimetry (ITC). Analysis of the ITC data reveals that drug/quadruplex binding stoichiometry is 1:1 for both interactions and that distamycin analog gives approximately a 10-fold increase in the quadruplex affinity.
Thermodynamic analysis of quadruplex DNA-drug interaction / Pagano, Bruno; C. A., Mattia; A., Virno; Randazzo, Antonio; Mayol, Luciano; Giancola, Concetta. - In: NUCLEOSIDES, NUCLEOTIDES & NUCLEIC ACIDS. - ISSN 1525-7770. - STAMPA. - 26:6-7(2007), pp. 761-765. [10.1080/15257770701499069]
Thermodynamic analysis of quadruplex DNA-drug interaction
PAGANO, BRUNO;RANDAZZO, ANTONIO;MAYOL, LUCIANO;GIANCOLA, CONCETTA
2007
Abstract
This work studies the binding properties of distamycin and its carbamoyl analog, containing four pyrrole units, with the [d(TGGGGT)](4) quadruplex by means of isothermal titration calorimetry (ITC). Analysis of the ITC data reveals that drug/quadruplex binding stoichiometry is 1:1 for both interactions and that distamycin analog gives approximately a 10-fold increase in the quadruplex affinity.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
