A physico-chemical characterization, based on NMR and CD spectroscopy, of quadruplexes formed by the oligonucleotide d(TGGGT), where two or three Gs are substituted by 8-bromo-2′-deoxyguanosine residues (dG Br ), is reported. The oligonucleotidic sequences d(TG Br G Br GT), d(TG Br GG Br T), d(TGG Br G Br T), and d(TG Br G Br G Br T) have been synthesized. Only sequences d(TG Br GG Br T) and d(TG Br G Br GT) were able to fold into a well defined quadruplex structure, and their CD profiles and thermal stabilities turned out to be very different from those observed for the natural counterpart, indicating that the 8-Br-dG residues dramatically affect the structure of the quadruplex.
Biophysical properties of Quadruplexes containing two or three 8-bromodeoxyguanosine residues / Petraccone, Luigi; I., Duro; Randazzo, Antonio; A., Virno; Mayol, Luciano; Giancola, Concetta. - In: NUCLEOSIDES, NUCLEOTIDES & NUCLEIC ACIDS. - ISSN 1525-7770. - STAMPA. - 26:6-7(2007), pp. 669-674. [10.1080/15257770701490589]
Biophysical properties of Quadruplexes containing two or three 8-bromodeoxyguanosine residues
PETRACCONE, LUIGI;RANDAZZO, ANTONIO;MAYOL, LUCIANO;GIANCOLA, CONCETTA
2007
Abstract
A physico-chemical characterization, based on NMR and CD spectroscopy, of quadruplexes formed by the oligonucleotide d(TGGGT), where two or three Gs are substituted by 8-bromo-2′-deoxyguanosine residues (dG Br ), is reported. The oligonucleotidic sequences d(TG Br G Br GT), d(TG Br GG Br T), d(TGG Br G Br T), and d(TG Br G Br G Br T) have been synthesized. Only sequences d(TG Br GG Br T) and d(TG Br G Br GT) were able to fold into a well defined quadruplex structure, and their CD profiles and thermal stabilities turned out to be very different from those observed for the natural counterpart, indicating that the 8-Br-dG residues dramatically affect the structure of the quadruplex.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
