Inhibition of erythroid differentiation in Mel cells: Cell cycle and DNA repair activity

Irradiation with a 3-s pulse of 254 nm UV light has been used to study sensitivity to mutagenic agents of mouse erythroleukemia (MEL) cell cultures in correlation with the cell cycle. A dose of UV irradiation was chosen that had no consequences for cell viability and growth. For this reason phenotypic effects were monitored on the progeny of all cells of the irradiated cultures by scoring those unable to undergo erythroid differentiation upon induction with dimethyl sulfoxide. The very short period of irradiation made it possible to show that MEL cells, synchronized by two sequential blocks of deoxythymidine and one of hydroxyurea (HU), are sensitive to UV irradiation only in a very short period of time at about 60 min after release from HU block. Determinations of deoxythymidine incorporation into DNA show that this time period corresponds only marginally to the initial part of the S phase during which irradiation has no consequences for cell properties. Cells are not sensitive to UV irradiation in G1 and in G2/M unless, immediately after irradiation and for the following 2 h, cultures are treated with 1 mM HU to interfere with DNA repair. Alkaline sucrose gradient analyses show at all tested times that irradiation leads to fragmentation of cell DNA. The data suggest that an immediate increase of deoxythymidine incorporation into DNA following irradiation is not necessary for the efficient repair of damaged DNA. In fact, the percent of cells expressing the erythroid phenotype is normal in the progeny of cells irradiated in G2/M, when TdR incorporation is at a minimum. Repair activities appear then to be mechanistically divided into two phases, (1) recognition labeling of the altered sites and (2) reconstitution of the DNA sequences. The first activity appears to be operative at all phases of the cycle, the second activity is little or not operative in G2/M, possibly delayed to the following G1 period.