In this study we have investigated the relationship between neointima formation and NF-κB activation in a model of endothelial denudation of rat carotid artery (balloon angioplasty) using the antioxidant pyrrolidine dithiocarbamate as inhibitor of NF-κB activation. Furthermore, we have correlated NF-κB activation to the expression of inducible isoforms of both nitric oxide synthase (iNOS) and cyclooxygenase (COX-2) in injured carotids. In control group a significant proliferation of neointima was observed 14 days after balloon angioplasty, which was correlated to an increase of NF-κB/DNA binding activity as well as p50/p65 nuclear levels compared to those observed in the carotids from naive or sham-operated rats. Furthermore, NF-κB activation was correlated to increased iNOS and COX-2, but not β-actin, protein expression. Treatment of rats for 14 days with the antioxidant agent pyrrolidine dithiocarbamate (50, 100, 200 mg/kg per os and day) caused a significant inhibition of all the parameters assayed, except β-actin protein expression. These results indicate that prevention of NF-κB activation may lead to the inhibition of neointima formation and suggest that antioxidant agents may have therapeutic relevance for the prevention of human restenosis.

Role of nuclear factor-kappaB in a rat model of vascular injury / Ialenti, Armando; Ianaro, Angela; Maffia, Pasquale; Carnuccio, Rosa; D'Acquisto, F.; Maiello, F. M.; DI ROSA, M.. - In: NAUNYN-SCHMIEDEBERG'S ARCHIVES OF PHARMACOLOGY. - ISSN 0028-1298. - STAMPA. - 364:(2001), pp. 343-350.

Role of nuclear factor-kappaB in a rat model of vascular injury.

IALENTI, ARMANDO;IANARO, ANGELA;MAFFIA, PASQUALE;CARNUCCIO, ROSA;
2001

Abstract

In this study we have investigated the relationship between neointima formation and NF-κB activation in a model of endothelial denudation of rat carotid artery (balloon angioplasty) using the antioxidant pyrrolidine dithiocarbamate as inhibitor of NF-κB activation. Furthermore, we have correlated NF-κB activation to the expression of inducible isoforms of both nitric oxide synthase (iNOS) and cyclooxygenase (COX-2) in injured carotids. In control group a significant proliferation of neointima was observed 14 days after balloon angioplasty, which was correlated to an increase of NF-κB/DNA binding activity as well as p50/p65 nuclear levels compared to those observed in the carotids from naive or sham-operated rats. Furthermore, NF-κB activation was correlated to increased iNOS and COX-2, but not β-actin, protein expression. Treatment of rats for 14 days with the antioxidant agent pyrrolidine dithiocarbamate (50, 100, 200 mg/kg per os and day) caused a significant inhibition of all the parameters assayed, except β-actin protein expression. These results indicate that prevention of NF-κB activation may lead to the inhibition of neointima formation and suggest that antioxidant agents may have therapeutic relevance for the prevention of human restenosis.
2001
Role of nuclear factor-kappaB in a rat model of vascular injury / Ialenti, Armando; Ianaro, Angela; Maffia, Pasquale; Carnuccio, Rosa; D'Acquisto, F.; Maiello, F. M.; DI ROSA, M.. - In: NAUNYN-SCHMIEDEBERG'S ARCHIVES OF PHARMACOLOGY. - ISSN 0028-1298. - STAMPA. - 364:(2001), pp. 343-350.
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11588/148401
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