The circular DNA decamer 5'-d < pCGC-TT-GCG-TT > 3' is studied in solution by means of NMR spectroscopy. At low temperature the molecule adopts a dumbbell structure with three Watson-Crick C-G base pairs and two two-residue loops in opposite parts of the molecule. On raising the temperature another conformer appears, in which the closing C-G base pair in the 5'-GTTC-3' loop is disrupted, whereas the opposite 5'-CTTG-3' loop remains stable. The two conformers are in slow equilibrium over a limited temperature range.
An NMR study of the conformation and thermodynamics of the circular dumbbell d<pCGC-TT-GCG-TT>. Slow exchange between two- and four-membered hairpin loops / Ippel, J. H.; Lanzotti, Virginia; Galeone, Aldo; Mayol, Luciano; VAN DEN BOOGART, J. E.; Pikkemaat, J. A.; Altona, C.. - In: JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS. - ISSN 0739-1102. - 9:5(1992), pp. 821-836. [10.1080/07391102.1992.10507961]
An NMR study of the conformation and thermodynamics of the circular dumbbell d. Slow exchange between two- and four-membered hairpin loops
LANZOTTI, VIRGINIA;GALEONE, ALDO;MAYOL, LUCIANO;
1992
Abstract
The circular DNA decamer 5'-d < pCGC-TT-GCG-TT > 3' is studied in solution by means of NMR spectroscopy. At low temperature the molecule adopts a dumbbell structure with three Watson-Crick C-G base pairs and two two-residue loops in opposite parts of the molecule. On raising the temperature another conformer appears, in which the closing C-G base pair in the 5'-GTTC-3' loop is disrupted, whereas the opposite 5'-CTTG-3' loop remains stable. The two conformers are in slow equilibrium over a limited temperature range.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
