ODNs containing a 3'-3' phosphodiester junction have been conveniently synthesized through a solid phase procedure involving only 3'-phosphoramidite nucleosides, starting from a modified support linking the first nucleotide through the base. Thermal denaturation studies showed that 16-mers with this inversion of polarity cooperatively bound to 5'-(purine)(m)(pyrimidine)(n)-3'-type duplexes by specific recognition of the oligopurine domains alternately on the two DNA strands. The nature of the 3'-3' junction and nearest neighbors affected the stability of the resulting triplexes.
Synthesis and triple helix formation by alternate strand recognition of oligonucleotides containing 3'-3' phosphodiester bonds / DE NAPOLI, Lorenzo; A., Messere; Montesarchio, Daniela; A., Pepe; Piccialli, Gennaro; Varra, Michela. - In: JOURNAL OF ORGANIC CHEMISTRY. - ISSN 0022-3263. - ELETTRONICO. - 62:(1997), pp. 9024-9030. [10.1021/jo970589t]
Synthesis and triple helix formation by alternate strand recognition of oligonucleotides containing 3'-3' phosphodiester bonds
DE NAPOLI, LORENZO;MONTESARCHIO, DANIELA;PICCIALLI, GENNARO;VARRA, MICHELA
1997
Abstract
ODNs containing a 3'-3' phosphodiester junction have been conveniently synthesized through a solid phase procedure involving only 3'-phosphoramidite nucleosides, starting from a modified support linking the first nucleotide through the base. Thermal denaturation studies showed that 16-mers with this inversion of polarity cooperatively bound to 5'-(purine)(m)(pyrimidine)(n)-3'-type duplexes by specific recognition of the oligopurine domains alternately on the two DNA strands. The nature of the 3'-3' junction and nearest neighbors affected the stability of the resulting triplexes.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
