A convenient 'on line' solid-phase synthesis of oligonucleotides conjugated at the 3'-end with peptides by means of a polymeric support linking the first nucleoside via the base has been developed. A 17-mer designed for antisense experiments against HIV-1, linking at the 3'-terminus the tripeptide Gly-Gly-His, was prepared in good yields and characterized by MALDI-TOF mass spectrometry.
A new solid-phase synthesis of oligonucleotides 3’-conjugated with peptides / DE NAPOLI, Lorenzo; A., Messere; Montesarchio, Daniela; Piccialli, Gennaro; E., Benedetti; E., Bucci; Rossi, Filomena. - In: BIOORGANIC & MEDICINAL CHEMISTRY. - ISSN 0968-0896. - ELETTRONICO. - 7:(1999), pp. 395-400. [10.1016/S0968-0896(98)00250-8]
A new solid-phase synthesis of oligonucleotides 3’-conjugated with peptides
DE NAPOLI, LORENZO;MONTESARCHIO, DANIELA;PICCIALLI, GENNARO;ROSSI, FILOMENA
1999
Abstract
A convenient 'on line' solid-phase synthesis of oligonucleotides conjugated at the 3'-end with peptides by means of a polymeric support linking the first nucleoside via the base has been developed. A 17-mer designed for antisense experiments against HIV-1, linking at the 3'-terminus the tripeptide Gly-Gly-His, was prepared in good yields and characterized by MALDI-TOF mass spectrometry.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
