A new sample preparation method for matrix-assisted laser desorption/ionization (MALDI) analysis of native rough-type lipopolysaccharides (R-type LPSs) is presented. In our MALDI mass spectra, besides the [M--H](-) ions, abundant ions originating from the cleavage between the 3-deoxy-D-manno-oct-2-ulosonic acid (Kdo) unit and the lipid A moiety are always present, giving important pieces of information about the structure of the molecules analyzed. Remarkably, in most cases, the comparison of the MALDI mass spectra of the intact R-type LPS with the O-deacylated one allowed us to obtain the structure of the lipid A moiety
New conditions for matrix-assisted laser desorption/ionization mass spectrometry of native bacterial R-type lipopolysaccharides / L., Sturiale; D., Garozzo; Silipo, Alba; Lanzetta, Rosa; Parrilli, Michelangelo; Molinaro, Antonio. - In: RAPID COMMUNICATIONS IN MASS SPECTROMETRY. - ISSN 0951-4198. - STAMPA. - 19:13(2005), pp. 1829-1834.
New conditions for matrix-assisted laser desorption/ionization mass spectrometry of native bacterial R-type lipopolysaccharides
SILIPO, ALBA;LANZETTA, ROSA;PARRILLI, MICHELANGELO;MOLINARO, ANTONIO
2005
Abstract
A new sample preparation method for matrix-assisted laser desorption/ionization (MALDI) analysis of native rough-type lipopolysaccharides (R-type LPSs) is presented. In our MALDI mass spectra, besides the [M--H](-) ions, abundant ions originating from the cleavage between the 3-deoxy-D-manno-oct-2-ulosonic acid (Kdo) unit and the lipid A moiety are always present, giving important pieces of information about the structure of the molecules analyzed. Remarkably, in most cases, the comparison of the MALDI mass spectra of the intact R-type LPS with the O-deacylated one allowed us to obtain the structure of the lipid A moietyFile | Dimensione | Formato | |
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