During recent years, quantitative proteome profiling has taken advantage of incorporating the traditional stable isotope dilution analysis into global scale or discovery-based proteomic experiments that use mass spectrometers as detectors to allow the pairwise study of differently expressed proteins. Quantitative protein analysis by means of the isotope-coded affinity tag (ICAT) method and tandem mass spectrometry (MS) enables the pairwise comparison of protein expression levels in biological samples. Herein, a modified ICAT reagent, named BAAICAT (beta-alanine-arm-ICAT) in which the polyether linker is replaced by a more water-soluble polyamide one, was investigated.
Synthesis and proteomic activity evaluation of a new isotope-coded affinity tagging (ICAT) reagent / Guaragna, Annalisa; Amoresano, Angela; Pinto, V; Monti, Gianluca; Mastrobuoni, G; Marino, Gennaro; Palumbo, Giovanni. - In: BIOCONJUGATE CHEMISTRY. - ISSN 1043-1802. - STAMPA. - 19:5(2008), pp. 1095-1104. [10.1021/bc800010b]
Synthesis and proteomic activity evaluation of a new isotope-coded affinity tagging (ICAT) reagent.
GUARAGNA, ANNALISA
;AMORESANO, ANGELA;MONTI, GIANLUCA;MARINO, GENNARO;PALUMBO, GIOVANNI
2008
Abstract
During recent years, quantitative proteome profiling has taken advantage of incorporating the traditional stable isotope dilution analysis into global scale or discovery-based proteomic experiments that use mass spectrometers as detectors to allow the pairwise study of differently expressed proteins. Quantitative protein analysis by means of the isotope-coded affinity tag (ICAT) method and tandem mass spectrometry (MS) enables the pairwise comparison of protein expression levels in biological samples. Herein, a modified ICAT reagent, named BAAICAT (beta-alanine-arm-ICAT) in which the polyether linker is replaced by a more water-soluble polyamide one, was investigated.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
