1. Surface antigens of B. bubalis spermatozoa were solunilized by Triton X-100 and EDTA; 2. Two major polypeptides, immunoprecipitated from the seminal plasma by the antibodies against the sperm extract, exhibited the same electrophoretic mobilities of two immunorelated sperm surface antigens; 3. The two polypeptides were isolated from the seminal plasma, by a multi-step chromatographic procedure, and found subunits of a single protein (MW 30,000, called SP 30; 4. The SP 30 protein bound in vitro to the postacrosomal region of homologous spermatozoa from cauda epididymis; 5. The localization of the sperm-coating antigen on the cell surface is compatible with a role in the fertilization process.
Detection of a sperm-coating antigen in the semen of Bubalus bubalis / P., Bergamo; Venditti, Paola; Sansone, Giovanni; L., Ferrara; Abrescia, Paolo. - In: COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY. B, COMPARATIVE BIOCHEMISTRY. - ISSN 0305-0491. - STAMPA. - 102B:(1992), pp. 733-742. [10.1016/0305-0491(92)90072-y]
Detection of a sperm-coating antigen in the semen of Bubalus bubalis
VENDITTI, PAOLA;SANSONE, GIOVANNI;ABRESCIA, PAOLO
1992
Abstract
1. Surface antigens of B. bubalis spermatozoa were solunilized by Triton X-100 and EDTA; 2. Two major polypeptides, immunoprecipitated from the seminal plasma by the antibodies against the sperm extract, exhibited the same electrophoretic mobilities of two immunorelated sperm surface antigens; 3. The two polypeptides were isolated from the seminal plasma, by a multi-step chromatographic procedure, and found subunits of a single protein (MW 30,000, called SP 30; 4. The SP 30 protein bound in vitro to the postacrosomal region of homologous spermatozoa from cauda epididymis; 5. The localization of the sperm-coating antigen on the cell surface is compatible with a role in the fertilization process.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
