It is known that Caprine Herpesvirus 1 (CpHV-1) causes apoptosis in mitogen-stimulated as well as not stimulated caprine peripheral blood mononuclear cells (PBMC). Initial experiments in Madin Darby bovine kidney (MDBK) cells revealed that CpHV-1 infection induced apoptotic features like chromatin condensation and DNA laddering. Thus, to characterize in more detail this apoptotic process, activation of caspase-8, -9 and -3 in MDBK cells CpHV-1 infected was investigated and demonstrated. In addition, CpHV-1 infection resulted in disruption of mitochondrial membrane potential, cytochrome c release and alterations in the pro- and anti-apoptotic proteins of Bcl-2 family. Proteolytic cleavage of poly(ADP-ribose) polymerases (PARP), confirming the activation of downstream caspases, was also observed. Our data indicated that a "cross-talk" between the death-receptor (extrinsic) pathway and the mitochondrial (intrinsic) pathway occurred in CpHV-1-induced apoptosis in vitro
Analysis of apoptosis induced by Caprine Herpesvirus 1 in vitro / Longo, Mariangela; Fiorito, Filomena; Marfè, G; Montagnaro, Serena; Pisanelli, Giuseppe; DE MARTINO, Luisa; Iovane, Giuseppe; Pagnini, Ugo. - In: VIRUS RESEARCH. - ISSN 0168-1702. - ELETTRONICO. - 18:145(2009), pp. 227-235. [10.1016/j.virusres.2009.07.008]
Analysis of apoptosis induced by Caprine Herpesvirus 1 in vitro.
LONGO, MARIANGELA;FIORITO, FILOMENA;MONTAGNARO, SERENA;PISANELLI, GIUSEPPE;DE MARTINO, LUISA;IOVANE, GIUSEPPE;PAGNINI, UGO
2009
Abstract
It is known that Caprine Herpesvirus 1 (CpHV-1) causes apoptosis in mitogen-stimulated as well as not stimulated caprine peripheral blood mononuclear cells (PBMC). Initial experiments in Madin Darby bovine kidney (MDBK) cells revealed that CpHV-1 infection induced apoptotic features like chromatin condensation and DNA laddering. Thus, to characterize in more detail this apoptotic process, activation of caspase-8, -9 and -3 in MDBK cells CpHV-1 infected was investigated and demonstrated. In addition, CpHV-1 infection resulted in disruption of mitochondrial membrane potential, cytochrome c release and alterations in the pro- and anti-apoptotic proteins of Bcl-2 family. Proteolytic cleavage of poly(ADP-ribose) polymerases (PARP), confirming the activation of downstream caspases, was also observed. Our data indicated that a "cross-talk" between the death-receptor (extrinsic) pathway and the mitochondrial (intrinsic) pathway occurred in CpHV-1-induced apoptosis in vitroFile | Dimensione | Formato | |
---|---|---|---|
Analysis of apoptosis induced by Caprine Herpesvirus 1 in vitro.pdf
solo utenti autorizzati
Tipologia:
Documento in Post-print
Licenza:
Accesso privato/ristretto
Dimensione
598.06 kB
Formato
Adobe PDF
|
598.06 kB | Adobe PDF | Visualizza/Apri Richiedi una copia |
I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.