We report on tests of a combined fluorescence and radionuclide planar imaging system for in vivo investigation on small animals. Combined images of anaesthetized mice bearing a surface solid tumor are presented. The fluorescent marker is a hematoporphyrin compound laser-excited with green light and imaged in the red fluorescence emission with a standard monochrome charge coupled device (CCD) camera. The gamma-ray (99mTc) pinhole imaging system uses a semiconductor pixel detector obtained by hybridizing a Silicon (300-μm thick) or a CdTe (1-mm thick) pixel detector to the Medipix2 (55-μm pitch) readout integrated circuit for single photon counting. The acquisition of combined images of the tumor area (fluorescence: animal top view; radionuclide: bottom view) shows that the tumor area can be imaged in a few minutes, with a few millimeter resolution (1-mm pinhole diameter), radioactively (99mTc MIBI, 74 MBq), and with the optical system. Combined imaging revealed also a different uptake of the two types of tumors studied (one grown from anaplastic human thyroid carcinoma-derived cells, the other from human papillary carcinoma-derived cells). Future progress will be toward a more compact optical setup and the use of a thicker CdTe detector.
Experimental Study on In Vivo Optical and Radionuclide Imaging in Small Animals / Autiero, M.; Celentano, Luigi; Cozzolino, R.; Laccetti, P.; Marotta, M.; Mettivier, Giovanni; Montesi, M. C.; Riccio, P.; Roberti, G.; Russo, Paolo. - In: IEEE TRANSACTIONS ON NUCLEAR SCIENCE. - ISSN 0018-9499. - STAMPA. - 52 (1):2005, 52 (1): 205-209(2005), pp. 205-209. [10.1109/TNS.2004.843095]
Experimental Study on In Vivo Optical and Radionuclide Imaging in Small Animals
CELENTANO, LUIGI;METTIVIER, GIOVANNI;M. C. Montesi;G. Roberti;RUSSO, PAOLO
2005
Abstract
We report on tests of a combined fluorescence and radionuclide planar imaging system for in vivo investigation on small animals. Combined images of anaesthetized mice bearing a surface solid tumor are presented. The fluorescent marker is a hematoporphyrin compound laser-excited with green light and imaged in the red fluorescence emission with a standard monochrome charge coupled device (CCD) camera. The gamma-ray (99mTc) pinhole imaging system uses a semiconductor pixel detector obtained by hybridizing a Silicon (300-μm thick) or a CdTe (1-mm thick) pixel detector to the Medipix2 (55-μm pitch) readout integrated circuit for single photon counting. The acquisition of combined images of the tumor area (fluorescence: animal top view; radionuclide: bottom view) shows that the tumor area can be imaged in a few minutes, with a few millimeter resolution (1-mm pinhole diameter), radioactively (99mTc MIBI, 74 MBq), and with the optical system. Combined imaging revealed also a different uptake of the two types of tumors studied (one grown from anaplastic human thyroid carcinoma-derived cells, the other from human papillary carcinoma-derived cells). Future progress will be toward a more compact optical setup and the use of a thicker CdTe detector.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
