The structure of the carbohydrate backbone of the core-lipid A region of the lipooligosaccharide (LOS) of the alkaliphilic and slightly halophilic bacterium Halomonas sp. has been elucidated. The LOS was fully deacylated, dephosphorylated, and reduced at the free reducing end. The structure, obtained by means of compositional analysis, 2D NMR spectroscopy, and MALDI mass spectrometry, was determined as the following: All sugars are D-pyranoses; Hep is L-glycero-D-manno-heptose and Kdo is 3-deoxy-D-manno-oct-2-ulosonic acid.
The structure of the carbohydrate backbone of the lipooligosaccharide from an alkaliphilic Halomonas sp / Silipo, Alba; Gargiulo, Valentina; Sturiale, L.; Marchetti, R.; Prizeman, P.; Grant, W. D.; DE CASTRO, Cristina; Garozzo, D.; Lanzetta, Rosa; Parrilli, Michelangelo; Molinaro, Antonio. - In: CARBOHYDRATE RESEARCH. - ISSN 0008-6215. - STAMPA. - 345:(2010), pp. 1971-1975. [10.1016/j.carres.2010.06.007]
The structure of the carbohydrate backbone of the lipooligosaccharide from an alkaliphilic Halomonas sp
SILIPO, ALBA;GARGIULO, VALENTINA;R. Marchetti;DE CASTRO, CRISTINA;LANZETTA, ROSA;PARRILLI, MICHELANGELO;MOLINARO, ANTONIO
2010
Abstract
The structure of the carbohydrate backbone of the core-lipid A region of the lipooligosaccharide (LOS) of the alkaliphilic and slightly halophilic bacterium Halomonas sp. has been elucidated. The LOS was fully deacylated, dephosphorylated, and reduced at the free reducing end. The structure, obtained by means of compositional analysis, 2D NMR spectroscopy, and MALDI mass spectrometry, was determined as the following: All sugars are D-pyranoses; Hep is L-glycero-D-manno-heptose and Kdo is 3-deoxy-D-manno-oct-2-ulosonic acid.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
