The thrombin-binding aptamer (TBA) is a consensus DNA 15-mer that binds specifically to human [alpha]-thrombin at nanomolar concentrations and inhibits its procoagulant functions. Recently, a modified TBA (mTBA) containing a 5'-5' inversion-of-polarity site has been shown to be more stable and to possess a higher thrombin affinity than its unmodified counterpart. The structure of the thrombin-TBA complex has previously been determined at low resolution, but did not provide a detailed picture of the aptamer conformation or of the protein-DNA assembly, while that of the complex with mTBA is unknown. Crystallographic analysis of the thrombin-mTBA complex has been attempted. The crystals diffracted to 2.15 Å resolution and belonged to space group I222.
Crystallization and Preliminary X-Ray Crystallographic Analysis of the Complex of Human Alpha Thrombin With A Modified Thrombin Binding Aptamer / RUSSO KRAUSS, Irene; Merlino, Antonello; Randazzo, Antonio; Mazzarella, Lelio; Sica, Filomena. - In: ACTA CRYSTALLOGRAPHICA. SECTION F, STRUCTURAL BIOLOGY AND CRYSTALLIZATION COMMUNICATIONS. - ISSN 1744-3091. - ELETTRONICO. - 66:(2010), pp. 961-963. [10.1107/S1744309110024632]
Crystallization and Preliminary X-Ray Crystallographic Analysis of the Complex of Human Alpha Thrombin With A Modified Thrombin Binding Aptamer
RUSSO KRAUSS, IRENE;MERLINO, ANTONELLO;RANDAZZO, ANTONIO;MAZZARELLA, LELIO;SICA, FILOMENA
2010
Abstract
The thrombin-binding aptamer (TBA) is a consensus DNA 15-mer that binds specifically to human [alpha]-thrombin at nanomolar concentrations and inhibits its procoagulant functions. Recently, a modified TBA (mTBA) containing a 5'-5' inversion-of-polarity site has been shown to be more stable and to possess a higher thrombin affinity than its unmodified counterpart. The structure of the thrombin-TBA complex has previously been determined at low resolution, but did not provide a detailed picture of the aptamer conformation or of the protein-DNA assembly, while that of the complex with mTBA is unknown. Crystallographic analysis of the thrombin-mTBA complex has been attempted. The crystals diffracted to 2.15 Å resolution and belonged to space group I222.File | Dimensione | Formato | |
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