The transcription start site and promoter of the rat gene coding for the transcription factor NF-1 have been identified. The NF-1 promoter was fused to the chloramphenicol acetyltransferase-coding sequence, and the resulting plasmid was transcriptionally active in the HepG2 cell line. Footprinting and gel retardation analysis indicated that the transcription factor Sp1 binds to the NF-1 promoter. Mutants in the Sp1-binding site displayed a strong reduction in transcriptional activity.
Transcription of the promoter of the rat NF-1 gene depends on the integrity of an Sp1 recognition site / Ammendola, Rosario; F., Gounari; G., Piaggio; DE SIMONE, Vincenzo; Cortese, Riccardo. - In: MOLECULAR AND CELLULAR BIOLOGY. - ISSN 0270-7306. - STAMPA. - 10:(1990), pp. 387-390.
Transcription of the promoter of the rat NF-1 gene depends on the integrity of an Sp1 recognition site
AMMENDOLA, ROSARIO;DE SIMONE, VINCENZO;CORTESE, RICCARDO
1990
Abstract
The transcription start site and promoter of the rat gene coding for the transcription factor NF-1 have been identified. The NF-1 promoter was fused to the chloramphenicol acetyltransferase-coding sequence, and the resulting plasmid was transcriptionally active in the HepG2 cell line. Footprinting and gel retardation analysis indicated that the transcription factor Sp1 binds to the NF-1 promoter. Mutants in the Sp1-binding site displayed a strong reduction in transcriptional activity.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
