CONTEXT: A subset (approximately 3%-5%) of patients with cystic fibrosis (CF) develops severe liver disease with portal hypertension. OBJECTIVE: To assess whether any of 9 polymorphisms in 5 candidate genes (alpha(1)-antitrypsin or alpha(1)-antiprotease [SERPINA1], angiotensin-converting enzyme [ACE], glutathione S-transferase [GSTP1], mannose-binding lectin 2 [MBL2], and transforming growth factor beta1 [TGFB1]) are associated with severe liver disease in patients with CF. DESIGN, SETTING, AND PARTICIPANTS: Two-stage case-control study enrolling patients with CF and severe liver disease with portal hypertension (CFLD) from 63 CF centers in the United States as well as 32 in Canada and 18 outside of North America, with the University of North Carolina at Chapel Hill as the coordinating site. In the initial study, 124 patients with CFLD (enrolled January 1999-December 2004) and 843 control patients without CFLD were studied by genotyping 9 polymorphisms in 5 genes previously studied as modifiers of liver disease in CF. In the second stage, the SERPINA1 Z allele and TGFB1 codon 10 genotype were tested in an additional 136 patients with CFLD (enrolled January 2005-February 2007) and 1088 with no CFLD. MAIN OUTCOME MEASURES: Differences in distribution of genotypes in patients with CFLD vs patients without CFLD. RESULTS: The initial study showed CFLD to be associated with the SERPINA1 Z allele (odds ratio [OR], 4.72; 95% confidence interval [CI], 2.31-9.61; P = 3.3 x 10(-6)) and with TGFB1 codon 10 CC genotype (OR, 1.53; 95% CI, 1.16-2.03; P = 2.8 x 10(-3)). In the replication study, CFLD was associated with the SERPINA1 Z allele (OR, 3.42; 95% CI, 1.54-7.59; P = 1.4 x 10(-3)) but not with TGFB1 codon 10. A combined analysis of the initial and replication studies by logistic regression showed CFLD to be associated with SERPINA1 Z allele (OR, 5.04; 95% CI, 2.88-8.83; P = 1.5 x 10(-8)). CONCLUSIONS: The SERPINA1 Z allele is a risk factor for liver disease in CF. Patients who carry the Z allele are at greater risk (OR, approximately 5) of developing severe liver disease with portal hypertension.
Genetic modifiers of liver disease in cystic fibrosis / Bartlett, Jr; Friedman, Kj; Ling, Sc; Pace, Rg; Bell, Sc; Bourke, B; Castaldo, Giuseppe; Castellani, C; Cipolli, M; Colombo, C; Colombo, Jl; Debray, D; Fernandez, A; Lacaille, F; Macek M., Jr; Rowland, M; Salvatore, Francesco; Taylor, Cj; Wainwright, C; Wilschanski, M; Zemková, D; Hannah, Wb; Phillips, Mj; Corey, M; Zielenski, J; Dorfman, R; Wang, Y; Zou, F; Silverman, Lm; Drumm, Ml; Wright, Fa; Lange, Em; Durie, Pr; Knowles, Mr; Collaborators: Clancy JP, Gene Modifier Study G. r. o. u. p.; Sindel, Lj; Roberts, Dm; Roberts, V; Radford, Pj; Argel, N; Morgan, Wj; Douthit, Jl; Schellhase, De; Anderson, P; Taggart, A; Morrissey, B; Platzker, Ac; Woo, Ms; Fukushima, L; Hsu, E; Shay, Gf; Hardy, Ka; Moss, Rb; Dunn, Ce; Pian, Ms; Wojtczak, Ha; Burns, L; Henig, Nr; Nielson, Dw; Landon, C; Thompson, A; Accurso, Fj; Nick, Ja; Jones, M; Lapin, C; Drapeau, Vm; Egan, Me; Padman, R; Winnie, Gb; George, C; Olson, El; Light, Mj; Geller, De; Gondor, M; Flanary, J; Stecenko, Aa; Guill, Mf; Mccolley, Sa; Mccolley, Sa; Potter, Em; Chung, Y; Garvey, M; Howenstine, Ms; Sannuti, A; Yeley, J; Sloven, Dg; Ahrens, Rc; Teresi, M; Riva, Cm; Davis, S; Quiniones Ellis, B; Gabor, C; Lever, Tf; Welch, R; Cairns, A; Corrigan, M; Zeitlin, Pl; Brass, L; Dorkin, H; Levy, H; Huntington, I; O'Sullivan, Bp; Simon, Rh; Nasr, Sz; Lumeng, N; Ball, Me; Toder, Ds; Honicky, Re; Fitch, S; Contreras, L; Regelmann, We; Phillips, Jr; Mcnamara, J; Johnson, M; Ruiz, Fe; Adcock, Kg; Konig, P; Black, P; Weigel, Jd; Noyes, Be; Kociela, Vl; Ferkol T., Jr; Boyle, M; Colombo, Jl; Brascia, T; Parker, Hw; Zanni, Rl; Fiel, Sb; Lomas, P; Taylor Cousar, J; Borowitz, D; DeCelie Germana, Jk; Cohen, R; Gannon, M; Dimango, Ea; Mencin, Aa; Lobritto, Sj; Benitez, M; Walker, Pa; Berdella, Mn; Langfelder Schwind, E; Ren, Cl; Rovitelli, Ak; Anbar, Rd; Lindner, Dm; Perciaccante, Rg; Dozor, Aj; Knowles, Mr; Leigh, Mw; Taylor Cousar, J; Voynow, Ja; Auten, Kj; Schechter, Ms; Omlor, Gj; Ouellette, Da; Karp, Cl; Joseph, Pm; Konstan, Mw; Mccoy, Ks; Royce, F; Bartosik, S; Vauthy, Pa; Vauthy, Ml; Kramer, Jc; Hensel, S; Perez, Cr; Thomas, Nj; Hess, Jc; Holsclaw, Ds; Scanlin, Tf; Rubenstein, R; Murray, C; Skotleski, M; Fiel, Sb; Sexauer, Wp; Ko, A; Hillman, J; Orenstein, Dm; Schechter, Ms; Flume, Pa; Brown, D; Schoumacher, R; Culbreath, B; Moore, Pe; Slovis, B; Dambro, N; Garbarz, J; Hiatt, Pw; Olivier, Kn; Amaro, R; Macleod, L; Liou, Tg; Froh, Dk; Epstein, Ce; Schmidt, J; Elliot, G; Williams, R; Anderson, M; Gadd, J; Gibson, Rl; Mcnamara, S; Worrell, K; Moskowitz, Sm; Mccarthy, M; Llewellyn, C; Wicks, S; Moffett, Ks; Baer, Ls; do Pico, Ga; Makholm, Lm; Rock, Mj; Osmond, Sr; Biller, J; Miller, T; Fernandez, A; Renteria, F; Bell, Sc; Wainwright, C; Lewindon, P; Selvadurai, H; Gaskin, K; Van Biervliet, S; Montgomery, M; Rabin, Hr; Leong, J; Zuberbuhler, P; Brown, Ne; Tabak, J; Davidson, Ag; Nakielna, Em; Habbick, B; Waters, I; Wiltse, S; Kepron, W; Pasterkamp, H; Garey, Dn; Bishop, G; Noseworthy, M; Michael, Rt; Dale, Am; Gosse, Fa; Robinson, W; Durie, Pr; Corey, M; Freitag, A; Pedder, L; Van Wylick, R; Lougheed, Md; Kodiattu, L; Jackson, M; Malhotra, K; Lyttle, B; Paterson, Na; Aaron, S; Boland, M; Kovesi, T; Smith, A; Kumar, Vj; Zinger, S; Tullis, E; Simard, F; Rivard, L; Cantin, A; Cote, G; Lands, Lc; Marcotte, Je; Matouk, E; Berthiaume, Y; Jeanneret, A; Van Spall, M; Rivard, G; Boucher, J; Petit, N; Holmes, B; Cotton, D; Ramlall, K; Repetto, G; Macek M., Jr; Vavrova, V; Bartosova, J; Fila, L; Debray, D; Lacaille, F; Munck, A; Tümmler, B; Rowland, M; Bourke, B; Canny, G; Gallagher, C; Rivlin, J; Picard, E; Blau, H; Wilschanski, M; Springer, C; Kerem, E; Yahav, Y; Bujanover, Y; Casciaro, R; Colombo, C; Castaldo, G; Salvatore, F; Raia, Valeria; Cipolli, M; Castellani, C; Sinaasappel, M; Dooijes, D; Ling, Sc; Kayserova, H; Ozcelik, U; Kiper, N; Dogru, D; Taylor, Cj; Mcgaw, J.. - In: JAMA. - ISSN 0098-7484. - ELETTRONICO. - 302:(2009), pp. 1076-1083. [10.1001/jama.2009.1295]
Genetic modifiers of liver disease in cystic fibrosis.
CASTALDO, GIUSEPPE;SALVATORE, FRANCESCO;RAIA, VALERIA;
2009
Abstract
CONTEXT: A subset (approximately 3%-5%) of patients with cystic fibrosis (CF) develops severe liver disease with portal hypertension. OBJECTIVE: To assess whether any of 9 polymorphisms in 5 candidate genes (alpha(1)-antitrypsin or alpha(1)-antiprotease [SERPINA1], angiotensin-converting enzyme [ACE], glutathione S-transferase [GSTP1], mannose-binding lectin 2 [MBL2], and transforming growth factor beta1 [TGFB1]) are associated with severe liver disease in patients with CF. DESIGN, SETTING, AND PARTICIPANTS: Two-stage case-control study enrolling patients with CF and severe liver disease with portal hypertension (CFLD) from 63 CF centers in the United States as well as 32 in Canada and 18 outside of North America, with the University of North Carolina at Chapel Hill as the coordinating site. In the initial study, 124 patients with CFLD (enrolled January 1999-December 2004) and 843 control patients without CFLD were studied by genotyping 9 polymorphisms in 5 genes previously studied as modifiers of liver disease in CF. In the second stage, the SERPINA1 Z allele and TGFB1 codon 10 genotype were tested in an additional 136 patients with CFLD (enrolled January 2005-February 2007) and 1088 with no CFLD. MAIN OUTCOME MEASURES: Differences in distribution of genotypes in patients with CFLD vs patients without CFLD. RESULTS: The initial study showed CFLD to be associated with the SERPINA1 Z allele (odds ratio [OR], 4.72; 95% confidence interval [CI], 2.31-9.61; P = 3.3 x 10(-6)) and with TGFB1 codon 10 CC genotype (OR, 1.53; 95% CI, 1.16-2.03; P = 2.8 x 10(-3)). In the replication study, CFLD was associated with the SERPINA1 Z allele (OR, 3.42; 95% CI, 1.54-7.59; P = 1.4 x 10(-3)) but not with TGFB1 codon 10. A combined analysis of the initial and replication studies by logistic regression showed CFLD to be associated with SERPINA1 Z allele (OR, 5.04; 95% CI, 2.88-8.83; P = 1.5 x 10(-8)). CONCLUSIONS: The SERPINA1 Z allele is a risk factor for liver disease in CF. Patients who carry the Z allele are at greater risk (OR, approximately 5) of developing severe liver disease with portal hypertension.| File | Dimensione | Formato | |
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