One of the most important goals in the fields of biology and medicine is the possibility to dispose of efficient tools for the characterization of the extraordinary complexity of ligand-receptor interactions. To approach this theme, we explored the use of crystalline silicon (cSi) technology for the realization of a biotechnological device in which the ligand-receptor interactions are revealed by means of optical measurements. Here, we describe a chemical procedure for the functionalization of microwell etched on silicon wafers, and the subsequent anchoring of biologicalmolecules like an antibody anti-A20murine lymphoma cell line. The optical analysis of the interaction on the biochips between the bound biomolecule and their corresponding ligand indicated that the functionalized cSi is suitable for this application.
Silicon-Based Technology for Ligand-ReceptorMolecular Identification / Lamberti, Annalisa; Sanges, Carmen; Migliaccio, Nunzia; L., De Stefano; I., Rea; E., Orabona; G., Scala; I., Rendina; Arcari, Paolo. - In: JOURNAL OF ATOMIC, MOLECULAR, AND OPTICAL PHYSICS. - ISSN 1687-9228. - 2012:(2012), pp. 1-5. [10.1155/2012/948390]
Silicon-Based Technology for Ligand-ReceptorMolecular Identification
LAMBERTI, ANNALISA;SANGES, CARMEN;MIGLIACCIO, NUNZIA;ARCARI, PAOLO
2012
Abstract
One of the most important goals in the fields of biology and medicine is the possibility to dispose of efficient tools for the characterization of the extraordinary complexity of ligand-receptor interactions. To approach this theme, we explored the use of crystalline silicon (cSi) technology for the realization of a biotechnological device in which the ligand-receptor interactions are revealed by means of optical measurements. Here, we describe a chemical procedure for the functionalization of microwell etched on silicon wafers, and the subsequent anchoring of biologicalmolecules like an antibody anti-A20murine lymphoma cell line. The optical analysis of the interaction on the biochips between the bound biomolecule and their corresponding ligand indicated that the functionalized cSi is suitable for this application.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.