We investigate the interactions between proteins and DNA in solutions of living cells, triggered by UV, 200-fs laser pulses. DNA-protein crosslinking is obtained with two different techniques: conventional chemical or laser-induced. Our results evidence a nonlinear response of the cells to the laser irradiation in the investigated intensity range. The laser induced crosslink efficiency can reach a value approximately twice higher than that obtained with ordinary chemical methods. The experimental results are very well reproduced by a simple phenomenological model based on the interplay between two-photon absorption by DNA bases and cell damage induced by high intensity laser pulse.
Nonlinear protein – nucleic acid crosslinking induced byfemtosecond UV laser pulses in living cells / Altucci, Carlo; A., Nebbioso; R., Benedetti; Esposito, Rosario; V., Carafa; M., Conte; Micciarelli, Marco; L., Altucci; Velotta, Raffaele. - In: LASER PHYSICS LETTERS. - ISSN 1612-2011. - 9:3(2012), pp. 234-239. [10.1002/lapl.201110122]
Nonlinear protein – nucleic acid crosslinking induced byfemtosecond UV laser pulses in living cells
ALTUCCI, CARLO;ESPOSITO, ROSARIO;MICCIARELLI, MARCO;VELOTTA, RAFFAELE
2012
Abstract
We investigate the interactions between proteins and DNA in solutions of living cells, triggered by UV, 200-fs laser pulses. DNA-protein crosslinking is obtained with two different techniques: conventional chemical or laser-induced. Our results evidence a nonlinear response of the cells to the laser irradiation in the investigated intensity range. The laser induced crosslink efficiency can reach a value approximately twice higher than that obtained with ordinary chemical methods. The experimental results are very well reproduced by a simple phenomenological model based on the interplay between two-photon absorption by DNA bases and cell damage induced by high intensity laser pulse.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
