A chimeric cDNA was constructed, coding for the N-terminal region of BS-RNase (residues 1-49) and the C-terminal region of RNase A (residues 50-124). The resulting chimeric DNA was expressed in E.coli and found to code for RNase chains that spontaneously assembled into a covalently dimeric ribonuclease.
The Determinants of the Dimeric Structure of Seminal Ribonuclease are Located in its N-Terminal Region / DI DONATO, Alberto; Cafaro, Valeria; de Nigris, M.; Rizzo, M.; D'Alessio, Giuseppe. - In: BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS. - ISSN 0006-291X. - STAMPA. - 194:(1993), pp. 1440-1445.
The Determinants of the Dimeric Structure of Seminal Ribonuclease are Located in its N-Terminal Region
DI DONATO, ALBERTO;CAFARO, VALERIA;D'ALESSIO, GIUSEPPE
1993
Abstract
A chimeric cDNA was constructed, coding for the N-terminal region of BS-RNase (residues 1-49) and the C-terminal region of RNase A (residues 50-124). The resulting chimeric DNA was expressed in E.coli and found to code for RNase chains that spontaneously assembled into a covalently dimeric ribonuclease.File in questo prodotto:
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