Bovine seminal RNase (BS-RNase) is a dimeric member of the pancreatic-like ribonuclease superfamily, with antitumor activity. We have reported that recombinant Met(-1) BS-RNase is a less potent cytotoxic factor, while structurally and catalitically indistinguishable from BS-RNase isolated from natural sources. Mature recombinant BS-RNase instead displays full antitumor action. This suggests that the conformation of the N-terminal region of BS-RNase is among the structural determinants of its antitumor action, in addition to its catalytic activity and its quaternary structure.

Full antitumor action of recombinant seminal ribonuclease depends on the removal of its N-terminal methionine / Adinolfi, BIANCA STELLA; Cafaro, Valeria; D'Alessio, Giuseppe; DI DONATO, Alberto. - In: BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS. - ISSN 0006-291X. - STAMPA. - 213:(1995), pp. 525-532.

Full antitumor action of recombinant seminal ribonuclease depends on the removal of its N-terminal methionine

ADINOLFI, BIANCA STELLA;CAFARO, VALERIA;D'ALESSIO, GIUSEPPE;DI DONATO, ALBERTO
1995

Abstract

Bovine seminal RNase (BS-RNase) is a dimeric member of the pancreatic-like ribonuclease superfamily, with antitumor activity. We have reported that recombinant Met(-1) BS-RNase is a less potent cytotoxic factor, while structurally and catalitically indistinguishable from BS-RNase isolated from natural sources. Mature recombinant BS-RNase instead displays full antitumor action. This suggests that the conformation of the N-terminal region of BS-RNase is among the structural determinants of its antitumor action, in addition to its catalytic activity and its quaternary structure.
1995
Full antitumor action of recombinant seminal ribonuclease depends on the removal of its N-terminal methionine / Adinolfi, BIANCA STELLA; Cafaro, Valeria; D'Alessio, Giuseppe; DI DONATO, Alberto. - In: BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS. - ISSN 0006-291X. - STAMPA. - 213:(1995), pp. 525-532.
File in questo prodotto:
Non ci sono file associati a questo prodotto.

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11588/456300
Citazioni
  • ???jsp.display-item.citation.pmc??? ND
  • Scopus ND
  • ???jsp.display-item.citation.isi??? ND
social impact