Heparin conjugated silica nanoparticle synthesis

We report on a facile method towards a synthesis of novel hybrid heparin silica nanoparticles involving a modification of the Stober method. Tetra-Pr orthosilicate (TPOS) was used instead of the traditional TEOS or TMOS; by this way we could overcome the soly. problems of heparin in ethanol and exploit the good soly. of heparin in proper isopropanol-water mixts. Aminopropyl triethoxysilane (APTS) was also used to have a good link of heparin to silica particles. SEM, DLS, FTIR and NMR proved that we did find conditions in which heparin conjugated silica particles were produced.Thermogravimetry allowed to evaluate the heparin/silica wt. ratio to be 0.61. The efficiency of heparin binding to the particles was appreciated to be 35 wt.%.