A hydrophobic and catalyticmembrane has been obtained by grafting with γ-radiations a Teflonmembrane in presence of methylmethacrylate solution and by immobilizing on it, after treatment with glutaraldehyde, the β-Galactosidase enzyme. When employed in a non-isothermal bioreactor, the membrane showed an increase in catalytic activity proportional to the applied temperature gradients. The results have been explained in terms of distinct contributions from the process of thermodialysis and the conformational changes induced in the dynamic structure of the enzyme by the presence of heat flow. The increase of the yield of the process has been evaluated in terms of a coefficient α representing the percent increase of enzyme activity when a temperature difference of 1°C is applied across the membrane. The catalyticTeflonmembrane used here gave values of 20%, which are comparable to those obtained with other membrane systems, making this kind of membrane useful for practical applications in industrial processes.
Immobilization of beta-galactosidase on nylon membranes grafted with diethylenglycol dimethacrylate (DGDA) by gamma-radiation: Effect of membrane pore size / M. S., Mohy Eldin; DE MAIO, Anna; S., Di Martino; U., Bencivenga; S., Rossi; A., D'Uva; F. S., Gaeta; D. G., Mita. - In: ADVANCES IN POLYMER TECHNOLOGY. - ISSN 0730-6679. - ELETTRONICO. - 18:(1999), pp. 109-123. [10.1002/(SICI)1098-2329(199922)18:2<109::AID-ADV2>3.0.CO;2-3]
Immobilization of beta-galactosidase on nylon membranes grafted with diethylenglycol dimethacrylate (DGDA) by gamma-radiation: Effect of membrane pore size
DE MAIO, ANNA;
1999
Abstract
A hydrophobic and catalyticmembrane has been obtained by grafting with γ-radiations a Teflonmembrane in presence of methylmethacrylate solution and by immobilizing on it, after treatment with glutaraldehyde, the β-Galactosidase enzyme. When employed in a non-isothermal bioreactor, the membrane showed an increase in catalytic activity proportional to the applied temperature gradients. The results have been explained in terms of distinct contributions from the process of thermodialysis and the conformational changes induced in the dynamic structure of the enzyme by the presence of heat flow. The increase of the yield of the process has been evaluated in terms of a coefficient α representing the percent increase of enzyme activity when a temperature difference of 1°C is applied across the membrane. The catalyticTeflonmembrane used here gave values of 20%, which are comparable to those obtained with other membrane systems, making this kind of membrane useful for practical applications in industrial processes.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
