Abstract Two cyclic forms of the Enterobacterial Common Antigen were isolated from Escherichia coli O157:H−. These antigenic determinants were purified from the biomass through extensive chemical, enzymatic and chromatographic procedures whereas MALDI MS spectrometry indicated their cyclic nature with a polymerization degree of 4 or 5. The two species, denoted as ECACYC-4 and ECACYC-5, were assigned by NMR and showed no further substitution with other appendages such as acetyl groups as usually described for similar cyclic antigens from other Enterobacteriaceae
Occurrence and structure of cyclic Enterobacterial Common Antigen in Escherichia coli O157:H− / E., Fregolino; R., Ivanova; Lanzetta, Rosa; Molinaro, Antonio; Parrilli, Michelangelo; T., Paunova Krasteva; S. R., Stoitsova; DE CASTRO, Cristina. - In: CARBOHYDRATE RESEARCH. - ISSN 0008-6215. - 363:(2012), pp. 29-32. [10.1016/j.carres.2012.09.017]
Occurrence and structure of cyclic Enterobacterial Common Antigen in Escherichia coli O157:H−
LANZETTA, ROSA;MOLINARO, ANTONIO;PARRILLI, MICHELANGELO;DE CASTRO, CRISTINA
2012
Abstract
Abstract Two cyclic forms of the Enterobacterial Common Antigen were isolated from Escherichia coli O157:H−. These antigenic determinants were purified from the biomass through extensive chemical, enzymatic and chromatographic procedures whereas MALDI MS spectrometry indicated their cyclic nature with a polymerization degree of 4 or 5. The two species, denoted as ECACYC-4 and ECACYC-5, were assigned by NMR and showed no further substitution with other appendages such as acetyl groups as usually described for similar cyclic antigens from other Enterobacteriaceae| File | Dimensione | Formato | |
|---|---|---|---|
|
82_ECA.pdf
solo utenti autorizzati
Tipologia:
Altro materiale allegato
Licenza:
Accesso privato/ristretto
Dimensione
738.43 kB
Formato
Adobe PDF
|
738.43 kB | Adobe PDF | Visualizza/Apri Richiedi una copia |
I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
