This paper presents a mathematical model for a novel synthetic gene regulatory network. The aim of this circuit is to behave as a bistable switch for in vivo delivery of short hairpin RNA (shRNA) which can induce RNA interference (RNAi) of a target mRNA. Through the mathematical analysis performed here, it will be shown how the circuit can be controlled to induce sustained expression of a shRNA, with a transient input of two different inducer molecules.
Analysis and design of a versatile synthetic network for inducible gene expression in mammalian systems / A., Polynikis; G., Cuccato; S., Criscuolo; S. J., Hogan; DI BERNARDO, Mario; DI BERNARDO, Diego. - STAMPA. - (2010), pp. 182-185. (Intervento presentato al convegno 2010 Biomedical Circuits and Systems Conference (BioCAS) tenutosi a Paphos (Cipro) nel 3-5 novembre 2010) [10.1109/BIOCAS.2010.5709601].
Analysis and design of a versatile synthetic network for inducible gene expression in mammalian systems
DI BERNARDO, MARIO;DI BERNARDO, DIEGO
2010
Abstract
This paper presents a mathematical model for a novel synthetic gene regulatory network. The aim of this circuit is to behave as a bistable switch for in vivo delivery of short hairpin RNA (shRNA) which can induce RNA interference (RNAi) of a target mRNA. Through the mathematical analysis performed here, it will be shown how the circuit can be controlled to induce sustained expression of a shRNA, with a transient input of two different inducer molecules.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
